in order to reduce the costs associated with poly-β-hydroxybutyrate production, growth and poly-β-hydroxybutyrate production of ralstonia eutropha were studied in batch culture on different carbon sources. experiments were designed and conducted to not only lower the cost of poly-β-hydroxybutyrate production by using inexpensive substrates, but also to increase poly-β-hydroxybutyrate production...

the taguchi design of experiments was used to test the relative importance of medium components and environmental factors on poly(β-hydroxybutyrate)(phb) production by ralstonia eutropha. the optimum condition was obtained as: fructose concentration, 15 g/l; c/n ratio, 7.4; agitation speed 200 rpm; culture time, 40 h; temperature, 25 ° c; seed age, 15 h. at optimum condition the yield of phb pr...

thermodynamic study on the interaction of β-cd with poly ethylene oxide and poly acrylic acid was performed by isothermal titration calorimetry at 298k. when β-cd is added to the interpolymer complex, competition is created between host-guest and hydrogen bond. enthalpy of interaction between the β-cd and interpolymer complex was calculated using the extended solvation theory. p=1 shows that wi...

Thermodynamic study on the interaction of β-CD with poly ethylene oxide and poly acrylic acid was performed by isothermal titration calorimetry at 298K. when β-CD is added to the interpolymer complex, competition is created between host-guest and Hydrogen bond. Enthalpy of interaction between the β-CD and interpolymer complex was calculated using the extended solvation theory. P=1 Shows that w...

Terminally dibrominated poly(β-propiolactone) was prepared through sequentially ring-opening polymerization of β-propiolactone and dibromination the former product. Synthesis poly(β-propiolactone)-b poly(methyl methacrylate) tri-arm block copolymer achieved by atom transfer radical methyl methacrylate onto with bromide end groups. The characterization polymers carried out using 1H-NMR, DSC, TGA...

In Parkinson's disease (PD), neuronal cells undergo mitotic catastrophe and endoreduplication prior to cell death; however, the regulatory mechanisms remain to be defined. In this study, we investigated cell cycle regulation of DNA polymerase β (poly β) in rotenone-based dopaminergic cellular and animal models. Incubation with a low concentration (0.25 µM) of rotenone for 1.5 to 7 days resulted...

conclusions the results suggest that b. coagulans is a good candidate for the fermentative production of phb. background biodegradable polyesters are candidates for the development of environmental friendly plastics. poly-β-hydroxybutyrate (phb) is a type of polyester from the hydroxyalkanoates family, synthesized by bacteria as an intracellular material and accumulated as granules in the cytop...

BACKGROUND Retinoic acid-inducible gene-I (RIG-I) is a putative RNA helicase involved in immune reactions against RNA viruses and various inflammatory and autoimmune diseases. The purpose of the present study was to investigate the role of RIG-I in glomerular diseases. METHODS We treated human mesangial cells in culture with polyinosinic-polycytidylic acid (poly IC), which is an authentic dou...

The enzymatic ring-opening copolymerization of ε-caprolactone (ε-CL) and β-lactam by using Candida antarctica lipase B (CAL-B) as catalyst was studied. Variation of the feed ratios of 25:75, 50:50, and 75:25 of ε-CL/β-lactam was performed. The products contain poly(ε-CL-co-β-lactam) and the homopolymers of poly(ε-CL) and poly(β-lactam). The structure of the copolymers was determined by MALDI-To...

Retinoic acid inducible gene-I (RIG-I) is a member of the DExH box family of proteins. RIG-I acts as a sensor of viral infections through the recognition of viral double-stranded RNA (dsRNA). Recently, it was demonstrated that polyinosinic acid:polycytidylic acid [poly(I):poly(C)], a synthetic dsRNA analogue, induced the expression of RIG-I in various cell types, such as vascular endothelial ce...