Immunoreactive prolactin (ir-PRL) in rat brain has been consistently documented. However, the identity of this ir-PRL is controversial. Ir-PRL is defined by its ability to bind to PRL antibodies. All previous studies of brain ir-PRL have used polyclonal antibodies, at least one of which apparently crossreacts with a portion of the proopiomelanocortin molecule. To begin to define the epitopes co...

The objective of the present study was oriented to produce purified polyclonal antibody to prepare a prolactin solid phase coated tubes radioimmunoassay system. In the present study, production of polyclonal antibodies was carried out through immunization of three healthy white male mature New Zealand rabbits with a highly purified sheep prolactin antigen. The obtained anti-sera was purified us...

We used monoclonal antibodies to human IgG in a kinetic turbidimetric method with a centrifugal analyzer. Results for total IgG with monoclonal and polyclonal reagents correlated well (r = 0.979). Within- and between-batch CVs compared favorably (3.1 and 4.8%, respectively, for polyclonal antisera and 3.5 and 4.0%, respectively, for monoclonal reagents). The monoclonal antibody combination used...

To the Editor: Bioavailable testosterone is often derived from the free androgen index (1), which is reliant on measurements of both testosterone and sex hormone-binding globulin (SHBG). Although testosterone assays are widely available in various formats, assays for SHBG are more restricted partly because of the absence of automated procedures and the relatively high expense of kits. Dako (Den...

Urinary histoplasma antigen measurement can be useful for diagnosing systemic histoplasmosis and for monitoring treatment response, especially in immunocompromised patients. However, testing has traditionally been limited to specialized reference laboratories, as immunoassay reagents for the antigen were not widely available. Recently, a polyclonal-antibody-based in vitro diagnostic (IVD) kit f...

Antibodies bind other molecules strongly and specifically and are therefore useful as reagents in research, diagnosis and therapy. Antibodies taken from the blood of immunised animals are a mixture of different antibodies produced by different cells (they are described as polyclonal). Monoclonal antibodies are antibodies with a unique specificity, generally made by cloning cells containing a pa...