نتایج جستجو برای: polymerase chain reaction

تعداد نتایج: 681302  

Journal: :iranian journal of biotechnology 2014
zahra molavi choobini mohammad shadkhast hamdollah moshtaghi saeid habibian dehkordi homayon reza shahbazkia

background: genetic polymorphism of milk proteins has been associated with composition, manufacture, and traits of milk. caseins are the most important milk proteins whose genes are strongly linked and inherited as a raceme. κ-casein which is a quantitatively minor constituent of bovine milk is thought to play a critical role in organization, fixation and aggregation of casein micelles and firm...

Journal: :archives of razi institute 2016
m.h bozorgmehrifard m. hassanzdeh s. mirzaie m. banani

ornithobacterium rhinotracheale (ort) is a respiratory pathogen which has been isolated throughout the world from numerous bird species. the present study was designed to isolate and characterize the ort from domestic turkeys, quails and pigeons. for this purpose, 250 samples from each bird species (turkey, quail and pigeon) with or without respiratory signs were tested by taking of tracheal...

Journal: :archives of razi institute 2016
b. mosallanejad n. vakili r. avizeh m.r. seyfiabad shapouri m. pourmahdi

canine parvovirus type 2 (cpv-2) is one of the most common viruses responsible for acute hemorrhagic enteritis in dogs. a rapid and accurate diagnosis of cpv-2 infection is especially important in kennels in order to isolate infected dogs. the aim of the present study was to compare two laboratory tests i.e., polymerase change reaction (pcr) and immunochromatography assay (ica) most commonly us...

ژورنال: :مجله دانشکده پزشکی دانشگاه علوم پزشکی تهران 0
بیتا سلطانیان bita soltanian department of biology, science and research branch, islamic azad university, tehran, iran.گروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران شیوا ایرانی shiva irani department of biology, science and research branch, islamic azad university, tehran, iran.گروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات سروناز هاشمی sarvenaz hashemi human rabies vaccine laboratory, pasteurs production and research complex, institute of iran, tehran, iran.آزمایشگاه پژوهش و ساخت واکسن هاری انسانی، مجتمع تولیدی تحقیقاتی انستیتو پاستور ایران سید حمیدرضا مژگانی seyed hamid reza mozhgani human rabies vaccine laboratory, pasteurs production and research complex, institute of iran, tehran, iran. department of virology, faculty of public health, tehran university of medical sciences, tehran, iran.آزمایشگاه پژوهش و ساخت واکسن هاری انسانی، مجتمع تولیدی تحقیقاتی انستیتو پاستور ایران مهدی آجورلو mehdi ajorloo human rabies vaccine laboratory, pasteurs production and research complex, institute of iran, tehran, iran.آزمایشگاه پژوهش و ساخت واکسن هاری انسانی، مجتمع تولیدی تحقیقاتی انستیتو پاستور ایران یوسف چراغی yoosef cheraghi department of recombinant hepatitis b vaccine production, pasteurs production and research complex, institute of iran, tehran, iran.گروه تولید واکسن هپاتیت b نوترکیب، مجتمع تولیدی تحقیقاتی انستیتو پاستور ایران علیرضا غلامی

زمینه و هدف: در این مطالعه رده های سلولی vero آلوده به مایکوپلاسما، با رقت های مختلف سیپروفلوکساسین و انروفلوکساسین که مانع همانندسازی dna و سنتز پروتیین می شوند، تحت درمان قرار گرفتند. روش بررسی: در این پژوهش اکتشافی که در آزمایشگاه پژوهش و ساخت واکسن هاری انسانی انستیتو پاستور از آبان 1392 تا خرداد 1393انجام گردید، رقت های مختلف آنتی بیوتیکی مورد استفاده و مقایسه قرار گرفت. عملکرد این روش درم...

Journal: :hepatitis monthly 0
maryam moini gastroenterohepatology research center, shiraz university of medical sciences, shiraz, ir iran mazyar ziyaeyan professor alborzi clinical microbiology research center, shiraz university of medical sciences, shiraz, ir iran shapoor aghaei department of internal medicine, yasouj university of medical sciences, yasouj, ir iran mohammad mahdi sagheb nephrourology research center, shiraz university of medical sciences, shiraz, ir iran; nephrourology research center, shiraz university of medical sciences, shiraz, ir iran. tel: +98-7116474316, fax: +98-7116474316 seyed alireza taghavi gastroenterohepatology research center, shiraz university of medical sciences, shiraz, ir iran mahsa moeini professor alborzi clinical microbiology research center, shiraz university of medical sciences, shiraz, ir iran

results among the 181 anti-hcv ab negative patients, 13 (7.2%) were positive for hcv core ag and 11 (6%) had detectable hcv rna with a range of 40-336543 iu/ml by pcr. the two tests had a high measurement agreement (kappa=0.82, p<0.001). of the 13 patients with positive hcv core ag test results, 3 were negative for hcv rna. considering real time pcr for hcv rna as the gold standard for hcv infe...

Journal: :hepatitis monthly 0
bahman khalvati fahlyani diagnosis laboratory sciences and technology research center, school of paramedical, sciences shiraz university of medical sciences, shiraz, ir iran abbas behzad-behbahani diagnosis laboratory sciences and technology research center, school of paramedical, sciences shiraz university of medical sciences, shiraz, ir iran; department of biotechnology, school of paramedical sciences, shiraz university of medical sciences, shiraz, ir iran; diagnosis laboratory sciences and technology research center, school of paramedical, sciences shiraz university of medical sciences, shiraz, ir iran. tel: +98-7132270301, fax: +98-7132270301 seiied alireza taghavi gastroenterohepatology research center, shiraz university of medical sciences, shiraz, ir iran ali farhadi diagnosis laboratory sciences and technology research center, school of paramedical, sciences shiraz university of medical sciences, shiraz, ir iran; department of biotechnology, school of paramedical sciences, shiraz university of medical sciences, shiraz, ir iran saeede salehi diagnosis laboratory sciences and technology research center, school of paramedical, sciences shiraz university of medical sciences, shiraz, ir iran; department of biotechnology, school of paramedical sciences, shiraz university of medical sciences, shiraz, ir iran setare adibzadeh diagnosis laboratory sciences and technology research center, school of paramedical, sciences shiraz university of medical sciences, shiraz, ir iran; department of biotechnology, school of paramedical sciences, shiraz university of medical sciences, shiraz, ir iran

conclusions the quite sensitive in-house taqman real time rt-pcr assay was able to detect and quantify all four main hcv genotypes prevailing around all geographical regions of iran. results the lower limit detection of this in-house hcv real-time rt-pcr was determined as 100 rna copies/ml. inter- and intra-assay coefficient of variation (cv) of this in-house hcv real-time rt-pcr ranged from 0....

Journal: :iranian journal of virology 0
p mehrbod influenza unit pasteur institute of iran, tehran, iran e amini influenza unit pasteur institute of iran, tehran, iran m tavassoti-kheiri influenza unit pasteur institute of iran, tehran, iran

background and aims: influenza virus is the most important cause of annual morbidities and mortalities worldwide with numerous antigenic drifts and shifts. inaccessibility to effective drugs and vaccines has made world health authorities to be interested in traditional medicine in order to prevent spread of the infectious agent. garlic is one of the most famous of all plants in human history. i...

ژورنال: :مجله دانشکده پزشکی اصفهان 0
عبدالمجید فتی استاد، گروه انگل شناسی و قارچ شناسی، دانشکده ی پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران سید عبدالرحیم رضایی استاد، گروه ایمونولوژی، دانشکده ی پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران الهام مقدس استادیار، گروه انگل شناسی و قارچ شناسی، دانشکده ی پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران فائزه سادات موسوی وفا دانشجوی کارشناسی ارشد، گروه انگل شناسی و قارچ شناسی، دانشکده ی پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران سید علی اکبر شمسیان دانشیار، گروه انگل شناسی و قارچ شناسی، دانشکده ی پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران

مقدمه: leishmaniasis پوستی، از بیماری های انگلی پوستی است که در بسیاری مناطق جهان به ویژه ایران به صورت اندمیک وجود دارد. هر چند روش میکروسکوپی روش متداول تشخیص بیماری می باشد، اما تشخیص گونه های عامل leishmaniasis به صورت میکروسکوپی و یا از طریق یافته های بالینی امکان پذیر نیست. برای این منظور، روش های مولکولی از اهمیت ویژه ای برخوردار هستند. روش ها: در این مطالعه، شناسایی گونه ی عامل leishman...

Journal: :iranian journal of microbiology 0
yashgin hassanzadeh department of microbiology, science and research branch, islamic azad university, fars, iran. nima bahador department of microbiology, science and research branch, islamic azad university, fars, iran. majid baseri-salehi department of microbiology, kazeroun branch, islamic azad university, kazeroun, iran.

background and objective: photobacterium damselae subsp. damselae is a marine pathogenic bacterium which causes disease in marine animals and human. this bacterium mostly found in coastal shallow seawater. so, the aim of this study was isolation and characterization of photobacterium damselae subsp. damselae from edible fish of persian gulf, bandar abbas. material and methods: totally 100 fish ...

Journal: :jundishapur journal of microbiology 0
nader divan khosroshahi cellular and molecular research centre, qazvin university of medical sciences, qazvin, ir iran taghi naserpour farivar cellular and molecular research centre, qazvin university of medical sciences, qazvin, ir iran; cellular and molecular research centre, qazvin university of medical sciences, qazvin, ir iran. tel: +98-9128801401, fax: +98-2833324971. pouran johari cellular and molecular research centre, qazvin university of medical sciences, qazvin, ir iran

background legionellaceae contains legionella genus with over 52 species and 64 serogroups. it is one of the most important causes of respiratory disease in human. more than 30% of hospital-acquired pneumonia is caused by legionella. ventilator-associated pneumonia (vap) is an infection acquired in hospital wards, particularly in intensive care unit (icu). this disease approximately affects 9% ...

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