Different RNA species are rigorously discriminated and exported by distinct export factors, but this discrimination mechanism remains largely unknown. We previously showed, by RNA microinjection experiments, that intronless mRNAs are discriminated from U snRNAs based on their difference in RNA length. However, it was unclear how they are discriminated in the natural situation in which their nas...

The yeast Sac3:Cdc31:Sus1:Thp1 (TREX-2) complex facilitates the repositioning and association of actively transcribing genes with nuclear pores (NPCs)-"gene gating"-that is central to integrating transcription, processing, and mRNA nuclear export. We present here the crystal structure of Sus1 and Cdc31 bound to a central region of Sac3 (the CID domain) that is crucial for its function. Sac3(CID...

Export of mRNA from the nucleus is a central process in eukaryotic gene expression that has been implicated in several human diseases. Much of our understanding of how an mRNA is transported to the cytoplasm is derived from studies using yeast and fly models. We present here different mechanisms by which aberrant nuclear retention of mRNA can cause human disease. Emerging evidence that implicat...

In eukaryotic cells, the nuclear membrane creates a barrier between the nucleus and the cytoplasm. Whereas RNA synthesis occurs in the nucleus, they mostly function in the cytoplasm; thus export of RNA molecules from the nucleus to the cytoplasm is indispensable for normal function of the cells. The molecular mechanisms involved in each kind of cellular RNA export is gradually understood. The f...

To better understand carbon (C) cycling in arctic tundra we measured dissolved C production and export rates in mesocosms of three tundra vegetation types: tussock, inter-tussock and wet sedge. Three flushing frequencies were used to simulate storm events and determine potential mass export of dissolved C under increased soil water flow scenarios. Dissolved C production and export rates differe...

a diversification of the country’s export structure through the concerted development of its export-oriented manufacturing capacity could offer a viable alternative, as also stipulated in the country’s second socioeconomic and cultural development plan, by increasing the resilience of its economy against external shocks, supporting employment creation and technological innovation, and adding do...

raisin is the second major export item in iranian agricultural section after pistachio. in this study, effects of exchange rate fluctuations were investigated on quantity and price of exported raisin. therefore, data of 1970 to 2008 were reviewed by time series analysis and estimated by auto-regressive distributed lag model (ardl). results show the absence of a long-term relationship between ex...

Influenza remains a serious worldwide public health problem. After infection, viral genomic RNA is replicated in the nucleus and packed into viral ribonucleoprotein, which will then be exported to the cytoplasm via a cellular chromosome region maintenance 1 (CRM1)-dependent pathway for further assembly and budding. However, the nuclear export mechanism of influenza virus remains controversial. ...

Pro-inflammatory stimuli evoke an export of glutamate from microglia that is sufficient to contribute to excitotoxicity in neighbouring neurons. Since microglia also express various glutamate receptors themselves, we were interested in the potential feedback of glutamate on this system. Several agonists of mGluRs (metabotropic glutamate receptors) were applied to primary rat microglia, and the ...

While there is an increasing realization of the potential that IT offers for human welfare, IT-induced productivity and growth are confined to the developed world. It is argued that even though the international digital divide is a reality, there are certain specific characteristics of the new technology that leave scope for mitigating, if not totally bridging, the gap when appropriate policies...