trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. trypsin has been used widely in various biotechnological processes. in this study trypsin was purified using two precipitation cut, 35% and 75% as saturation percent of ammonium sulfate solutions, tca precipitation (2.5% w/v), ph precipitation and finally affinity chromatography. this m...

Selective precipitation of proteins can be used as a bulk method to recover the majority of proteins from a crude lysate, as a selective method to fractionate a subset of proteins from a protein solution, or as a very specific method to recover a single protein of interest from a purification step. This unit describes a number of methods suitable for selective precipitation. In each of the prot...

nowadays, undoubted access to up to date with high spatial resolution and low temporal resolution of meteorological parameters is a one of the necessary needs for meteorological science researches. in order to doing this research have been used the different daily, monthly and yearly iran precipitation from global and regional data base of gpcpøŒ gpccøŒ aphrodit v101 0.25øŒ aphrodit v101 0.5 ùˆ...

OBJECTIVE(S) The present study reports the development and validation of a sensitive and rapid extraction method beside high performance liquid chromatographic method for the determination of crocetin in human serum. MATERIALS AND METHODS The HPLC method was carried out by using a C18 reversed-phase column and a mobile phase composed of methanol/water/acetic acid (85:14.5:0.5 v/v/v) at the fl...

Regional climate models (RCMs) in general can simulate the characteristics of heavy/extreme precipitation more accurately than general circulation models (GCMs) as a result of more realistic representation of topography and mesoscale processes. An analogue method of statistical downscaling, which identifies the resolved large-scale atmospheric conditions associated with heavy precipitation, is ...

tio2 /fe3o4 nanocomposites with various tio2:fe3o4 ratios were synthesized by an ultrasonic-assisted deposition-precipitation method and their uv-light decolorization of congo dye and magnetic separation were investigated. the nanocomposite samples were characterized by scanning electron microscopy (sem), powder x-ray diffraction (xrd), surface area analayzer (bet) and fourier transform infrare...

Nano-structured lanthanum strontium cobalt ferrite, La0.6Sr0.4Co0.2Fe0.8O3 (LSCF), was successfully synthesized via co-precipitation method using metal nitrates as starting materials. Effects of precipitating agent and calcination temperature on the phase composition and morphology of synthesized powders were systematically studied using X-ray diffraction (XRD) and field emission scanning elect...

The synthesis of micro-sized, uniformly distributed Al2O3-15Vol% Ni powders were studied through three step co-precipitation of hydroxides mixtures from proper solution, calcination at air atmosphere and final step of calcined powders in a carbon bed. Al and Ni hydroxide and amorphous phase were first obtained from their salt’s solutions through chemical co-precipitation method by adjusti...