When washed at moderate temperatures, slices of many underground storage organs show a steady rise in their rate of respiration until it reaches a value two to five times that of freshly cut tissue. Laties (3) has recently reviewed our knowledge of these changes in respiration. In accordance with his suggestion, the respiration which develops gradually on washing will be referred to as induced ...

OBJECTIVES Costal cartilage is becoming increasingly popular as a graft source for facial reconstruction. However, carving methods have not changed in decades and continue to primarily rely on detailed maneuvers with a scalpel. There are few reports of mechanical devices for shaping costal cartilage, and to our knowledge their accuracy and precision have not been reported. We describe a simple ...

Acetaminophen (APAP) is an analgesic and antipyretic agent which may cause hepatotoxicity and nephrotoxicity with overdose in man and laboratory animals. In vivo studies suggest that in situ activation of APAP contributes to the development of nephrotoxicity. Associated with target organ toxicity is selective arylation of proteins, with a 58-kDa acetaminophen binding protein (58-ABP) being the ...

To determine the role of nitric oxide (NO) in rat liver transporter regulation, we investigated in precision-cut liver slices the effect of NO and peroxynitrite (ONOO(-)), a reaction product of NO with superoxide (O(2(-))), on mRNA levels of 13 influx and efflux transporters. To inactivate Kupffer cells (KCs), liver slices were prepared from rats treated with gadolinium chloride (Gd). Transport...

Arsenic is a known human carcinogen. These studies were designed to examine the impact of low arsenite concentrations on immediate early gene expression in precision-cut rat lung slices. Precision-cut lung slices are a versatile in-vitro system for toxicity studies, as they preserve the architecture and cellular heterogeneity of the lung. Since 0.1-100 microM arsenite did not compromise slice v...

Bioavailability and therapeutic outcome of treatment with HIV-protease inhibitors depends on intestinal and hepatic transporter-enzyme interplay. Liver transport of HIV protease inhibitors (saquinavir, darunavir) was assessed in the presence of aged garlic extract, because the HIV-infected often consume garlic supplements together with prescribed therapy. The in vitro uptake of both drugs into ...