The purpose of this study was to evaluate the effects of acidic primers on adhesive bonding to sintered aluminum oxide (alumina). Alumina disks were primed with one of the following materials: Acryl Bond, All Bond 2 Primer B, Alloy Primer, Estenia Opaque Primer, Eye Sight Opaque Primer, M.L. Primer, MR. Bond, and Super-Bond Liquid. Specimens were then bonded with an acrylic resin initiated with...

The results could be summarized as follow: 1Primer OP-B01 gave 7 monomorphic and 13 polymorphic fragments (65%). 2The Primer OP-B02 and Primer OP-B05 gave 4 monomorphic fragments with 14 polymorphic fragments (79%). 3The Primer HA-98 gave 4 monomorphic fragments with 19 detected polymorphic 83%. 4The Primer HA-99 and HB-12 gave 3 monomorphic fragments and 17 polymorphic 85 and 86%, respectively...

To provide feasible primer sets for performing a polymerase chain reaction (PCR) experiment, many primer design methods have been proposed. However, the majority of these methods require a relatively long time to obtain an optimal solution since large quantities of template DNA need to be analyzed. Furthermore, the designed primer sets usually do not provide a specific PCR product size. In rece...

Background: Genome walking is a DNA-cloning methodology that is used to isolate unknown genomic regions adjacent to known sequences. However, the existing genome-walking methods have their own limitations. Objectives: Our aim was to provide a simple and efficient genome-walking technology. Material and Methods: In this paper, we dev...

self etching primers, due to ease of manipulation, have been extensively used in recent years. these self etching primers containing a relatively weak acid, may not provide an optimal bond strength. the purpose of the present in-vitro investigation was to evaluate the effects of 37% phosphoric acid application prior to applying self etching primers on composite bond strength. in this experiment...

To detect five plant viruses (Beet black scorch virus, Beet necrotic yellow vein virus, Eggplant mottled dwarf virus, Pelargonium zonate spot virus, and Rice yellow mottle virus) for quarantine purposes, we designed 15 RT-PCR primer sets. Primer design was based on the nucleotide sequence of the coat protein gene, which is highly conserved within species. All but one primer set successfully amp...

The present study was conducted to evaluate the effect of acidic primers on adhesive bonding to sintered alumina. Alumina disk specimens were primed with one of the following acidic materials: Acryl Bond, All Bond II Primer B, Alloy Primer, Estenia Opaque Primer, Eye Sight Opaque Primer, M.L. Primer, MR. Bond, and Super-Bond Liquid. The disks were bonded with an adhesive resin (Super-Bond) init...

Retroviruses use a cellular tRNA molecule as primer for reverse transcription. The complementarity between the 3' end of this tRNA and a sequence near the 5' end of the viral RNA, the primer-binding site (PBS), allows the primer to anneal onto the viral RNA. During reverse transcription 18 nucleotides of the tRNA primer are copied into the viral cDNA, thereby regenerating the PBS sequence of th...

DNA polymerases amplify DNA fragments through primer extension reactions. However, polymerization behavior of short primers in the primer extension process has not been systematically explored. In this study, we examined the minimal primer length required for primer extension, and the effect of primer length, mismatches and other conditions on DNA polymerization using a non-radioactive method. ...