A sticky-flare gold nanoparticle probe (AuNP-probe) is designed by the combination of locked nucleic acid functionalized silencing microRNA technology for intracellular microRNA-21 (miRNA-21) sensitively detecting, fluorescence imaging, localizing and silencing. The limit detection as low 0.01 nM. Overexpressed miRNA-21 in cancer cells serves endogenous drug release stimuli to trigger probe-loa...

A “cocktail”of several probe drugs is often used to evaluate metabolic activity of multiple cytochrome P450 enzymes in one session. Some interactions among probe drugs can appear and may impact the rate of biotransformation of other ones. Our presented work was to aim on the influence of bupropion on rat cytochrome P450-mediated metabolism of tolbutamide. The biotransformation rates of tolbutam...

Understanding the potential for cytochrome P450 (P450)-mediated drug-drug interactions is a critical step in the drug discovery process. Although in vitro studies with CYP3A4, CYP2C9, and CYP2C19 have suggested the presence of multiple binding regions within the P450 active site based on probe substrate-dependent inhibition profiles, similar studies have not been performed with CYP2C8. The abil...

here we report a novel approach for preparation of a 6-day transdermal drug delivery system (tdds) as treatment for mild to moderate alzheimer’s disease. the spray drying method was used to prepare microparticles containing the anti-alzheimer drug. the content of the drug was determined by high performance liquid chromatography (hplc) method. the morphology and size range of the microparticles ...

drug resistance (especially multiple drug resistance) in mycobacterium tuberculosis makes global concerns in treatment and control of tuberculosis. rapid diagnosis of drug resistant strains of the bacteria has vital importance in the prognosis of the disease. the aim of this study was to identify the mutations responsible for drug resistance in mycobacterium tuberculosis strains derived from pa...

conclusions the mtbdrplus and mtbdrsl genotypic testing can serve as useful additional tools for dst in a high-burden country like pakistan provided it is used in combination with phenotypic testing. results the sensitivity of mtbdrplus for isoniazid and rifampicin was found to be 88.8% and 90.2%, respectively, while sensitivity of mtbdrsl for fluoroquinolones, amikacin/capreomycin, and ethambu...