The expression of inhibitory immune checkpoint molecules, such as programmed death-ligand (PD-L)1, is frequently observed in human cancers and can lead to the suppression T cell-mediated responses. Here, we apply expanded CRISPR-compatible (EC)CITE-seq, a technology that combines pooled CRISPR screens with single-cell mRNA surface protein measurements, explore molecular networks regulate PD-L1 ...

objective: breast cancer is the second leading cause of cancer death in women. cisplatin is a traditional cancer drug commonly used in chemotherapy for killing cancer cells. modulation at the mrna levels of apoptotic related genes often correlate with the sensitivity of various types of cancer cells to chemotherapeutic agents. nanoparticulate drug delivery systems are being developed to effecti...

Induction of the B7 family molecules by 12-O-tetradecanoyl phorbol 13-acetate (TPA) has been reported, however, the mechanism by which TPA up-regulates these molecules remains poorly understood. In this study, the expression of B7-DC, -H1, -H2, and -H3 in response to TPA was markedly induced in K562 cells. TPA also induced activation of ERK, p38 mitogen-activated protein kinase (MAPK), JNK, pho...