The membrane carrier for L-proline (product of the putP gene) of Escherichia coli K12 was solubilized and functionally reconstituted with E. coli phospholipid by the cholate dilution method. The counterflow activity of the reconstituted system was studied by preloading the proteoliposomes with either L-proline or the proline analogues: L-azetidine-2-carboxylate or 3,4-dehydro-L-proline. The dil...

  Abstract   Background: The polymorphic variants at codon 72 of the p53 gene, encoding either   proline or arginine at residue 72, produce marked change in the structure of p53.   From the evidence that the DNAmismatch repair system and p53 interact to maintain   genomic integrity, we hypothesized that the codon 72 variation may influence the   prevalence of microsatellite instability a featur...

evidence is emerging for the association of polymorphisms in prodh gene and increased risk of schizophrenia. in this project, peripheral blood sampling was obtained from 175 schizophrenia patients that their diseases were confirmed by psychiatrists. 185 healthy individuals were considered as control group. the related tests were administered on the basis of pcr-rflp. in the continuation, statis...

Exogenously provided proline has been shown to serve as an osmoprotectant in Bacillus subtilis. Uptake of proline is under osmotic control and functions independently of the known transport systems for the osmoprotectant glycine betaine. We cloned the structural gene (opuE) for this proline transport system and constructed a chromosomal opuE mutant by marker replacement. The resulting B. subtil...

Transforming growth factor-beta1 (TGF-beta 1) is a multifunctional cytokine that contributes to arterial remodelling by stimulating vascular smooth muscle cell (SMC) growth and collagen synthesis at sites of vascular injury. Since l-proline is essential for the synthesis of collagen, we examined whether TGF-beta 1 regulates the transcellular transport of l-proline by vascular SMCs. l-Proline up...

The effect of various NaCl treatments (0, 50, 100, 200 and 300 mM) at different growth and development stages (tillering, boot swelling, flowering and polination) of two wheat cultivars (Ghods : salt-sensitive; Boolani : salt-resistant) on proline concentration and the kinetic activity of proline dehydrogenase was studied under greenhouse conditions. Generally, in response to salinity treatment...

Among the twenty natural proteinogenic amino acids, proline is unique as its secondary amine forms a tertiary amide when incorporated into biopolymers, thus preventing hydrogen bond formation. Despite the lack of hydrogen bonds and thanks to conformational restriction of flexibility linked to the pyrrolidine ring, proline is able to stabilize peptide secondary structures such as b-turns or poly...

1. Recta of desert locusts were short-circuited and depleted of endogenous substrates by exposing them to saline containing cyclic AMP but no metabolites. Individual substrates were then added to substrate-depleted recta and the change in short-circuit current (/sc) monitored. 2. Proline or glucose (50 mil) caused by far the largest increase in 780 of all substrates tested. Stimulation of the 7...

1. It has been experimentally shown that breaking of the pyrrolidine ring of proline by nitrous acid is not a factor of sufficient magnitude to account for the amino nitrogen which is usually found in proline preparations. 2. A method for the preparation of racemic proline is described. The product was found to be free from amino nitrogen. 3. The dissociation constants of racemic proline and of...

the effects of lead on the proline content and dry weight of leaves and roots were investigated in two canola cultivars (brassica napus l.) grown in the hoagland solution. the growth of treated plants was inhibited under lead stress. lead induced differential accumulation of proline in canola grown in solution with the addition of 0, 100 and 200 mgl-1 of pb. hyola308 cultivar showed low biomass...