• EAEP increased extractability and bioaccessibility of soy isoflavone aglycones. phenolic compounds. resulted in extracts with higher antioxidant potential. improved the functional biological properties extracts. The effects enzymatic extraction strategies on extractability, bioconversion, biologically active aglycones, total content, activity aqueous from full-fat flour were evaluated. Protea...

introduction: proteolytic enzymes, specially collagenase, are used to digest extracellular matrix, cells isolation and primary culture. it is important to find new sources of plant or animal protease instead of bacterial or tissue collagenase. in the present research, actinidin, a plentiful protease in kiwifriut, was used to isolate human umbilical vein endothelial cells. materials and methods:...

Pseudomonas cepacia produces at least two extracellular proteases with apparent molecular masses of 36,000 and 40,000 Da. The 36-kDa protease has high proteolytic activity and the 40-kDa protease has low proteolytic activity with hide powder azure as a substrate. Monoclonal antibodies (MAbs) were raised against the purified 36- and 40-kDa proteases. Several MAbs directed against the 36-kDa prot...

it has already been reported that muscle necrosis induced by various phenylenediamine derivatives are correlated with their autoxidation rate. now in a more detailed investigation of the cytotoxic mechanism using a model system of isolated hepatocytes and ring-methylated structural isomer durenediamine (dd) we have shown that under aerobic conditions, phenylenediamine induced cytotoxicity and r...

probable use of chemical and biochemical detergents to wash polysulphone membranes using in milk ultrafiltration needs a multidisciplinary investigation. by increasing use of these membranes in food processing, washing and recovery of initial permeate flux, in order to use for a longer time from this technology, has much importance. in this research, influence of chemical detergents including e...

PURPOSE A Pseudomonas mutant deficient in protease IV has significantly reduced virulence in experimental keratitis. In the present study, the corneal toxicity of purified protease IV and its ability to augment the virulence of protease-IV-deficient bacteria were analyzed. METHODS The toxicity of purified protease IV was determined by intrastromally injecting the exoenzyme (20-200 ng) into th...

Guava (Psidium guajava L.), is adapted to an extensive range of soils, however, its development is strongly affected by salinity. Environmental stresses are of the major and influential factors in the amount of secondary metabolites, especially phenolic compounds present in plants. The purpose of this investigation was to study the leaf antioxidant and flavonoid contents (catechin, quercetin an...

entomopathogenic fungi produce a variety of degrading enzymes, including proteases, chitinases and lipases, to facilitate their entry through the massive barriers of insect cuticle. isolates of the entomopathogenic fungi vary considerably in their proteolytic activity and virulence. the proteolytic activity of different isolates has been hypothesized to reflect their virulence toward the host. ...