In this feeding trial, a total of 500 Cobb-500 broiler (day-old) chickens were randomly assigned to control dietary treatment (basal diet only) or supplemented with crushed seeds coriander (Coriandrum sativum L.), black cumin (Bunium persicum (Boiss.) B. Fedtsch) and ajwain (Carum copticum L.) (at 10 mg/kg each) exogenous protease (30,000 IU/kg), respectively, in order assess the effect diets o...

Protease inhibitor Lopinavir, Ritonavir, Remdesivir yang bekerja pada RNA polimerase virus (Main Protease) diprediksi dapat memberikan aktivitas baik dalam pengikatan dengan reseptor secara in silico dan potensial sebagai penghambat COVID-19. Tujuan penelitian ini adalah untuk mengetahui toksisitas Remdesivir, Favipiravir menggunakan software Toxtree interaksi kestabilan obat resepetor M-Protea...

PURPOSE To determine the distribution of the protease IV gene, the production of this and other proteases by multiple strains of Pseudomonas, and the virulence of a mutant specifically deficient in protease IV. METHODS The protease IV gene was cloned, its sequence analyzed, and its chromosomal location determined by pulse-field gel electrophoresis. Three PCR reactions were used to detect the ...

Objective: Investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (SMF). Methods: Optimum temperature for enzyme activity in the crude extract was 40 ◦C at a pH between 8.0 and 9.0. The studies on pH stability showed that the enzyme...

In the course of a search for an alkaline stable protease for industrial use, an alkaline protease (protease BYA) was isolated from an alkalophilic Bacillus sp. Y, and its properties were characterized. Its optimum pH was pH 10.0-12.5, when casein was used as a substrate. In addition to the stability of protease BYA at pH 6.5-13.0, it was also very stable towards various surface-active agents, ...

It has already been reported that muscle necrosis induced by various phenylenediamine derivatives are correlated with their autoxidation rate. Now in a more detailed investigation of the cytotoxic mechanism using a model system of isolated hepatocytes and ring-methylated structural isomer durenediamine (DD) we have shown that under aerobic conditions, phenylenediamine induced cytotoxicity and R...

Molecular docking analysis is routinely used in modern drug research to understand and predict the relationship between a molecule target protein from microbe. The entry replication of pathogens host cells can be prevented by drugs identified this way. coronavirus disease associated with SARS-CoV-2, COVID-19, has become today's most infectious lethal pandemic world. Burgeoning absence any parti...

Dengue fever is an infectious disease caused by the dengue virus, and there no yet effective drug to treat this successfully. Our study aimed identify bioactive compounds of Acorus calamus L. its potential role in inhibiting virus NS3 protease-helicase. Liquid Chromatography-Mass Spectrometry analyzed phytochemical constituents. Drug-likeness predominant compound methanol extract was investigat...