human alpha 1-antitrypsin (aat) cdna was obtained from hepg2 cell lines. after pcr and construction of expression vector ppiczα-aat, human aat was expressed in the yeast pichia pastoris (p.pastoris) in a secretary manner and under the control of inducible alcohol oxidase 1 (aox1) promoter. the amount of aat protein in medium was measured as 60 mg/l 72 hr after induction with methanol. results i...

production of thermostable alkaline protease in tray bioreactor for solid state fermentation is a unique process which may lead to an economical approach for production of enzyme from agricultural waste or low cost substrate. in solid state fermentation using tray bioreactor, several agricultural residues such as rice bran, sugarcane bagasse, wheat bran, barely bran, corn meal and corn husk wer...

the effect of a neuropeptide, the tachykinin on α-amylase, protease and lipase activities in the midgut of american cockroach, periplaneta americana (linnaeus) is evaluated . immunohistochemical reactivities against tachykinin (tachykinin- ir) were detected in the midgut. tachykinin-ir cells in three regions (anterior, median and posterior) of the midgut were counted in normally fed, starved fo...

background and objectives: proteases are a group of enzymes that catalyze the degradation of proteins resulting in the pro- duction of their amino acid constituents. they are the most important group of industrial enzymes which account for about 60% of total enzymes in the market and produced mainly by microorganisms. the attempts were made to study the kinetic parameters of protease produced b...

HIGHLIGHTS Boceprevir is a potential therapeutic that targets the main protease of SARS-CoV-2. Mutations change inhibitory binding pattern in catalytic domain protease. SARS-CoV-2 cause decrease boceprevir affinity.

The aim of this study was to determine the inhibitory effects of feed ingredients on protease activities of Argyrosomus regius larvae using in vitro techniques. A. regius larvae fed on a commercial feeding procedure were sampled thirteen times, during the sampling period (from 3 to 32 days after hatching (DAH)). The differences observed in protease activities of meagre larvae during the samplin...

the goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native iranian microorganisms. at the end of screening program, a bacillus subtilis bp-36 strain producing thermophilic alkaline protease was isolated from a hot spring in ardebil province. the target enzyme was purified using a one-step aqueous two-phase systems (atps) prot...

objective: investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (smf). methods: optimum temperature for enzyme activity in the crude extract was 40 ◦c at a ph between 8.0 and 9.0. the studies on ph stability showed that the enzyme was stab...

background and objectives: psychrotrophic bacteria can produce enzymes at low temperatures; this provides a wide biotechnological potential, and offers numerous economical advantages over the use of mesophilic bacteria. in this study, extracellular protease production by psychrotrophic rheinheimera sp. (km459533) was optimized by the response surface methodology. materials and methods: the cult...

background and objectives: bacteria, most prevalently the pseudomonas species possess high capacity to utilize and de- grade petroleum hydrocarbons and are classified as the hydrocarbonoclastic microorganisms. many species of the genus pseudomonas are notorious for their aerobic degradation capacity, extracellular enzyme production and are metabolically versatile organisms capable of utilizing ...