We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility during chromatographic separation. We show this efficiently detects both integral membrane and memb...

Dysfunctional protein complexes are often associated with diseases. To develop effective treatments it is essential to understand the composition, formation and functionality of protein complexes. Novel techniques like complexome profiling give an overview of possible protein-protein interactions in an entire sample. In this approach intact protein complexes are separated using blue-native elec...

Plants synthesize diverse diterpenoids with numerous functions in organ development and stress resistance. However, the role of glandular trichome (GT) GT-localized biosynthesis plants remains unknown. Here, identification 10 diterpene synthases (diTPSs) revealed diversity diterpenoid Artemisia annua. Protein–protein interactions (PPIs) between AaKSL1 AaCPS2 plastids highlighted their potential...

The reliable production of large amounts of stable, high-quality proteins is a major challenge facing pharmaceutical protein biochemists, necessary for fulfilling demands from structural biology, for high-throughput screening, and for assay purposes throughout early discovery. One strategy for bypassing purification challenges in problematic systems is to engineer multiple forms of a particular...

introduction: tuberculosis (tb) remains a deadly infectious disease despite all the efforts to reduce its incidence. spread of multidrug resistant tb has seriously undermined the efforts to control the disease globally. in this study protein expression profile of mdr and sensitive isolates of mtb were analyzed and compared in order to identify proteins, which could be used in prevention, diagno...

MALDI profiling and imaging mass spectrometry (IMS) are novel techniques for direct analysis of peptides and small proteins in biological tissues. In this work we applied them to the study of Anopheles gambiae antennae, with the aim of analysing expression of soluble proteins involved in olfaction perireceptor events. MALDI spectra obtained by direct profiling on single antennae and by the anal...

PURPOSE Ovarian cancer is a devastating disease and biomarkers for its early diagnosis are urgently required. Serum may be a valuable source of biomarkers that may be revealed by proteomic profiling. Herein, complementary serum protein profiling strategies were employed for discovery of biomarkers that could discriminate cases of malignant and benign ovarian cancer. EXPERIMENTAL DESIGN Identi...

Functional links between genes can be predicted using phylogenetic profiling, by correlating the appearance and loss of homologs in subsets of species. However, effective genome-wide phylogenetic profiling has been hindered by the large fraction of human genes related to each other through historical duplication events. Here, we overcame this challenge by automatically profiling over 30,000 gro...

The recent dramatic improvements in high-resolution mass spectrometry (MS) have revolutionized the speed and scope of proteomic studies. Conventional MS-based proteomics methodologies allow global protein profiling based on expression levels. Although these techniques are promising, there are numerous biological activities yet to be unveiled, such as the dynamic regulation of enzyme activity. C...

the need for clean groundwater resources to have sustainable development in a country is undoubted. due to the importance and high quality of karstic waters in supplying water in iran especially in shahrood city, it is attempted in this research work to recognize and explore karstic waters in southwest of tepal area, shahrood. for this purpose, integration of the results obtained from the metho...