Immature embryos of Cymbidium iridioides were successfully cultured on five different media with various supplements and maintained in three different light conditions. Within three week of culture, embryos formed nodular structures. Cultures under full light condition formed green protocorm-like bodies while, other light conditions failed to support optimum germination. Immature embryos of 10 ...

A critical step in the development of Agrobacterium tumefaciens-mediated transformation in orchid genus is the establishment of optimal conditions for T-DNA gene delivery into protocorm-like bodies from which the whole plantlets can be regenerated. As a first step in the development of a successful Agrobacterium tumefaciens-mediated transformation for Dendrobium savin white, factors influencing...

carbon and nitrogen are the two elements that most affect plant organogenesis. in vitro, usually as part of the macronutrients. some anecdotal evidence from the literature suggests that the ammonium (nh4+) to nitrate (no3–) ratio may affect orchid organogenesis. in this study, to test this hypothesis, different nh4+: no3– ratios were tested on the development of protocorm-like bodies (plbs) of ...

Carbon and nitrogen are the two elements that most affect plant organogenesis. In vitro, usually as part of the macronutrients. Some anecdotal evidence from the literature suggests that the ammonium (NH4+) to nitrate (NO3–) ratio may affect orchid organogenesis. In this study, to test this hypothesis, different NH4+: NO3– ratios were tested on the development of protocorm-like bodies (PLBs) of ...

Auxin flows in a polar manner to target tissues and exert its morphogenic effect. Preventing auxin movement, with polar auxin transport (PAT) inhibitors, or increasing auxin levels in tissues through exogenous application can provide a means for assessing the importance of appropriate tissue distribution and concentration of this hormone during development. The formulation of culture media for ...