Small, Gram-negative bacteria that produced a blue-green fluorescence characteristic of Pseudomonas aeruginosa (Bacillus pyocyaneus) were found in all but 2 cases of an unusually severe outbreak of bovine mastitis involving 23 cows. Since the outbreak wais unusual in character, a study was carried on to determine the identity of the organisms and their possible significance. The results are rep...

In this study, hyperspectral fluorescence imaging techniques were investigated for detection of microbial biofilm on stainless steel plates typically used to manufacture food processing equipment. Stainless steel coupons were immersed in bacterium cultures of nonpathogenic E. coli, Pseudomonas pertucinogena, Erwinia chrysanthemi, and Listeria innocua. Following a 1-week growth in rich medium tr...

A multicenter evaluation was undertaken to evaluate the performance of a new three-color peptide nucleic acid fluorescence in situ hybridization assay that identifies isolates directly from blood cultures positive for Gram-negative bacilli (GNB). The assay correctly identified 100% (186/186) of the Escherichia coli isolates, 99.1% (109/110) of the Klebsiella pneumoniae isolates, and 95.8% (46/4...


 In the present investigation, antimicrobial activity of Catharanthus roseus (leaves and stem) was evaluated. The plant material extracted through maceration method results were recorded against different bacterial (Bacillus subtilis Pseudomonas flourescence) fungal (Aspergillus flavus Fusarium culmorum) strains. zones inhibition measured these strains compared with commercially available...

Objective(s) Some properties of neuraminidase produced by Pseudomonas aeruginosa PAO1 growth in a defined medium (BHI) were examined and evaluated for its features. Materials and Methods The obtained supernatant enzyme of P. aeruginosa PAO1 cultures was used in a sensitive fluorometric assay by using 2'-(4-methylumbelliferyl) a-D-N acetylneuraminic acid as substrate. As hydrolyzing MUN with ...

Zinc sulfide (ZnS) & zinc oxide (ZnO) nanoparticles was evaluated for their antimicrobial activity against four pathogenic strains. ZnS&ZnO nanoparticles were synthesized by simple aqueous chemical reaction in an aqueous solution. The main advantage of these nanoparticles (size of 10-30 nm) was that simply could be prepared by using cheap precursors in a cost effective and high throughput manne...

Abstract The conversion of soluble proteins into amyloid fibrils has importance in protein chemistry, biology, biotechnology and medicine. A novel lipase from Pseudomonas sp. was previously shown to have an extremely high aggregation propensity. It therefore herein studied elucidate the physicochemical structural determinants this extreme behaviour. Amyloid-like structures were found form sampl...

A pyrimidine-based sensor () has been synthesized through the facile one pot reaction of pyrrole-2-carboxaldehyde, 2-aminobenzimidazole and 1, 3-dimethylbarbituric acid in methanol using a few drops of HCl as a catalyst. Further, compound was fabricated into its fluorescent organic nanoparticles (FONPs) and characterized using DLS and SEM. The photophysical evaluation of the FONPs was done agai...

Phenazines, a group of fluorescent small molecules produced by the bacterium Pseudomonas aeruginosa, play a role in maintaining cellular redox homeostasis. Phenazines have been challenging to study in vivo due to their redox activity, presence both intra- and extracellularly, and their diverse chemical properties. Here, we describe a noninvasive in vivo optical technique to monitor phenazine co...