Hematological malignancies are treated with intensive high-dose chemotherapy, with or without radiation. This is followed by hematopoietic stem cell (HSC) transplantation (HSCT) to rescue or reconstitute hematopoiesis damaged by the anticancer therapy. Autologous HSC grafts may contain cancer cells and purging could further improve treatment outcomes. Similarly, allogeneic HSCT may be improved ...

Bulimia nervosa (BN) is one of the most frequent eating disorders in industrialized societies. Re-duced serotonin activity has been suggested to trigger some of the cognitive and mood distur-bances associated with BN. Therefore, pharmacological treatment of BN is mainly based on the use of selective serotonin reuptake inhibitors, that have proved effective. The biological basis of this disorder...

Two depuration, or purging, systems were examined for their effect on survival and evacuation Ž . rate of intestinal contents of the hindgut of crawfish Procambarus clarkii . In the immersion system, crawfish were held in baskets suspended in the water column and subjected to profuse aeration and low water exchange. Crawfish in the water spray system were maintained in shallow Ž . pools 1.3-cm ...

When compared to surface ecosystems, groundwater sampling has unique constraints, including limited access to ecosystems through wells. In order to monitor groundwater, a detailed understanding of groundwater biota and what biological sampling of wells truly reflects, is paramount. This study aims to address this uncertainty, comparing the composition of biota in groundwater wells prior to and ...

OBJECTIVE Early experiences of trauma or adverse events may be associated with eating disturbance later in life, but evidence is scarce. This study examined whether reported history of adverse life events predicted eating disturbance upon college entry and prospective changes over the first semester of college. METHOD First semester college students (n = 249) reported trauma/adverse event his...

Recent data suggest that tumor cells contaminating reinfused bone marrow may contribute to relapse in patients undergoing autologous bone marrow transplantation. Purging strategies that are able to remove these contaminating tumor cells need to be developed. This study describes how electroporation (EP) can be used to improve intracellular delivery of synthetic antisense oligodeoxynucleotides (...

This study explored the moderating effects of body dissatisfaction (BD) on the relationships between various constructs of control and weight restricting and control behaviours (WRCBs). Participants were 167 female undergraduates who completed self-report measures of control, BD and WRCBs. It was found that higher external locus of control (LOC) was related to less dieting and exercise and that...

Several studies have emphasized that inbreeding depression (ID) is enhanced under stressful conditions. Additionally, one might imagine a loss of adaptively plastic responses which may further contribute to a reduction in fitness under environmental stress. Here, we quantified ID in inbred families of the cyclical parthenogen Daphnia magna in the absence and presence of fish predation risk. We ...

Tumor cell contamination of clinical grafts is a major concern in autologous hematopoietic stem cell transplantation because these contaminating cells can contribute to relapse. In the present work, we use a suicide gene therapy approach that successfully accomplishes the two main goals of any purging strategy: highly efficient elimination of contaminating tumor cells and preservation of the en...

Autologous peripheral blood progenitor cell (PBPC) transplantation is the treatment of choice for selected myeloma patients. However, tumor cells contaminating the apheresis product are a potential source of relapse. Here we report a sequential purging strategy targeting mature and immature clonogenic myeloma cell populations in the autograft. Thawed PBPC products of myeloma patients were treat...