A study on skin cross-reactivity between stabilized "4C-labeled mycobacterial antigens, namely tuberculin purified protein derivative (PPD; from Mycobacterium tuberculosis), PPD-A (M. avium), PPD-Y (M. kansasii), PPD-G (M. scrofulaceum), PPD-B (M. intracellulare), and PPD-F (M. fortuitun), has been carried out in groups of guinea pigs sensitized with one of the following heat-killed mycobacteri...

Current recommendations for tuberculosis control are to screen high risk populations and provide chemoprophylaxis for those infected. In Edmonton, Alberta, one strategy has been to identify and provide TB skin tests to newly arrived immigrant school age children from TB endemic areas. The difficulty has been in identifying these children in the school population. This article describes a proces...

In the Caribbean region, the lifetime risk of active tuberculosis (TB) in purified protein derivative (PPD)-positive HIV-infected patients is 50%. Screening of individuals with cough who came to an HIV voluntary counseling and testing center in Haiti revealed active TB in 33% of patients. TB prophylaxis is effective in preventing active disease in HIV-infected individuals, and secondary prophyl...

Tuberculosis is a common disease in India. Mantoux test is used in children for diagnosis of tuberculosis. Seibert first prepared the purified protein derivative (PPD) from heat-concentrated old tuberculin by precipitating protein initially with trichloroacetic acid(l). PPD when diluted is adsorbed by glass and plastic surfaces. To minimize this a small amount of detergent. Tween-80, is added t...

BACKGROUND BCG vaccination of infants is thought to provide good protection in all settings. This study investigated whether Malawian infants made weaker responses across a cytokine panel after BCG vaccination, compared with UK infants. METHODS Diluted whole-blood samples were cultured with Mycobacterium tuberculosis purified protein derivative for 6 days from BCG-vaccinated infants 3 months ...

One hundred one subjects living in northwest Hunan Province, People's Republic of China (PRC) were tested with histolyn, (Berkeley Biological Laboratories), spherulin (Berkeley Biological Laboratories), and tubersol (PPD 5 tuberculin units, Connaught Laboratories). Age of the tested subjects ranged from 16 to 58 years; 93 subjects were 22 years old or younger. Nine subjects reacted to histolyn ...

A method has been described whereby tuberculin P P D is linked to formalinized erythrocytes by a coupling agent, tetrazotized benzidine (1). These antigen-coated erythrocytes are agglutinated by ant ibody to PPD. I t was observed that P P D could inhibit the hemagglutination more effectively than could polysaccharide I. I t appears, therefore, tha t the ant ibody concerned is directed toward th...

In 1957, Smith, Espir, Whitty, and Russell described what they regarded as characteristic changes in the spinal fluid of patients with multiple sclerosis following intrathecal injection of tuberculin (purified protein derivative, P.P.D.). They regarded these changes as indicating an abnormal and possibly specific immunological reaction, suggested that the administration of P.P.D. "might be bene...

Background: In Hong Kong there has been nearly universal neonatal BCG vaccination coverage since 1980. Method: 21 113 schoolchildren aged 6–9 years were skin tested with one unit of tuberculin (PPD RT-23) using the intradermal technique during a routine BCG revaccination programme. Information on sex, date of birth, date of tuberculin testing, and tuberculin reaction size at 72 hours was retrie...

The effect of Mycobacterium /eprae was studied on mitogen, PHA and ant igen, PPO-induced leucocyte migrat ion inhibit ion in 44 leprosy patients and 1 3 healthy controls using the leucocyte migrat ion inh ibi tion test . While M. /eprae decreased the PHA-generated inhibit ion of migrat ion of leucocytes in tuberculoid patients and healthy i ndividuals , i t enhanced the inh ibi tory effect on t...