background and aims: accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (fmd). in present study, was evaluated real time reverse transcription-polymerase chain reaction (rrt-pcr) assay along with diagnostic routine methods for the detection of all seven serotypes of fmd virus (fmdv), namely o, c, a, sat1, 2, 3 and asia 1 in biological sample...

The Eco Real-Time PCR system puts powerful qPCR applications within reach of individual researchers. Large, expensive thermal cyclers that take up an entire workspace are replaced with an affordable, compact system that fits easily on any lab bench (Figure 1). Delivering unsurpassed data quality in less than 40 minutes for 40-cycle runs, the Eco system revolutionizes qPCR accessibility for both...

About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs ( i.e., in real time). Data is therefore collected throughout the PCR process, rather than at the end of the PCR. This completely revolutionizes the way one approaches PCR-based quantitation of DNA and RNA. In real-time PCR, reactions are characterized by the point...

Real-time, fluorescence-based reverse transcription polymerase chain reaction (RT-PCR) has been transformed from an experimental technology into a mainstream scientific tool for the detection of RNA. This is because of several factors: 1) it is a homogeneous assay, which eliminates the requirement for post-PCR processing; 2) it has a wide dynamic range; 3) there is little interassay variation; ...