Research has demonstrated that swine feed can be a fomite for viral transmission and additives reduce contamination. Therefore, the objective of this study was to evaluate two in contaminated with PEDV or PRRSV. Feed included: no treatment, 0.33% commercial formaldehyde-based product, 0.50% medium chain fatty acids (MCFA) blend. samples were inoculated PRRSV alone together at an inoculation con...

The role of microRNAs (miRNAs) in how microbiota influence the host intestinal immune system is not fully understood. We compared expression profiles miRNAs and mRNAs lamina propria leukocytes (LPL) large intestines germ-free (GF) specific pathogen-free (SPF) mice. Microarray analysis revealed different between GF SPF Quantitative real time-PCR (qRT-PCR) showed that level miR-200 family members...

We report a case of Chagas disease reactivation in a patient with stage IIb follicular lymphoma in the cecum. He was admitted to the hospital with neutropenia and fever. He had a history of right hemicolectomy 6 months earlier and had received the sixth cycle of chemotherapy with cyclophosphamide/doxorubicin/vincristine/prednisone/rituximab. Blood and urine cultures were negative, but the fever...

Gene expression analysis by quantitative reverse transcription PCR (qRT-PCR) allows accurate quantifications of messenger RNA (mRNA) levels over different samples. Corrective methods for different steps in the qRT-PCR reaction have been reported; however, statistical analysis and presentation of substantially variable biological repeats present problems and are often not meaningful, for example...

Although the accuracy of quantitative real-time polymerase chain reaction (qRT-PCR) is highly dependent on the reliable reference genes, many commonly used reference genes are not stably expressed and as such are not suitable for quantification and normalization of qRT-PCR data. The aim of this study was to identify novel reliable reference genes in lung squamous-cell carcinoma. We used RNA seq...

The quantitative real-time polymerase chain reaction (qRT-PCR) approach has become a widely used method to analyze expression patterns of target genes. The selection of an optimal reference gene is a prerequisite for the accurate normalization of gene expression in qRT-PCR. The present study constitutes the first systematic evaluation of potential reference genes in Rhododendron molle G. Don. E...

In this study, we cloned and characterized three Manduca sexta odorant receptors (ORs). One receptor is a putative pheromone receptor expressed exclusively in a cell associated with male-specific type-I trichoid sensilla. We describe the results of real-time PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) experiments that show MsextaOR1 is expressed only in male antennae. In situ hybridiz...