Seneca Valley virus (SVV) is the causative agent of an emerging vesicular disease in swine, which is clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. In addition, SVV has been associated with neonatal mortality in piglets. While a commercial SVV qRT-PCR is available, commercial antibodies are lacking to diagnose SVV infections by immunohistochemistry (I...

Abstract The goal of this study was to demonstrate the functions and specific mechanism long non-coding RNA (lncRNA) GNAS-AS1 in lung adenocarcinoma. Levels lncRNA GNAS-AS1, microRNA (miR)-433-3p, Rab3A were assessed by quantitative real-time PCR (qRT-PCR). target-binding sites miR-433-3p, predicted confirmed bioinformatics tool (StarBase) a dual-luciferase reporter system. Cell proliferation a...

Abstract Purpose To validate a simplified RNA isolation method from biofabricating hydroxyapatite (HAp) scaffolds seeded with mesenchymal stem cells (MSCs) and to identify the appropriate reference gene. Methods Ten MSCs-HAp composites were used for by methods based on homogenization steps column-based purification procedures, while remaining (n = 13) was extracted traditional single-step metho...

The mechanism of hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) upregulation and its function in head neck squamous cell carcinoma (HNSCC) remains obscure. Herein, the expression HPRT1 underlying HNSCC were explored. Firstly, prognostic values simultaneously validated using bioinformatic analysis quantitative real-time PCR (qRT-PCR), immunohistochemistry staining with local samples. e...

Quantitative, reverse transcription, polymerase chain reaction (qRT-PCR) is facilitated by leveraging droplet microfluidic (DMF) system, which due to its precision dispensing and sample handling capabilities at microliter and lower volumes has emerged as a popular method for miniaturization of the PCR platform. This work substantially improves and extends the functional capabilities of our prev...

Synthetic linear SARS-CoV-2 RNA fragments (ssRNA) and a double-stranded DNA plasmid harbouring the nucleocapsid (N) gene are evaluated in four different VTM for up to 10 freeze–thaw cycles by qRT-PCR following magnetic bead extraction.

microRNA-422a (miR-422a) is downregulated in many hematopoietic tumors and solid including hepatocellular carcinoma. We previously demonstrated that hepatitis B virus X protein (HBx) expression of miR-422a HCC cell line HepG2 vitro. However, we explore the mechanisms underlying this action. Forkhead box proteins (FOX) G1/Q1/E1 are known to negatively regulate expression, prompted us determine w...

The golden pompano snout (GPS) and head kidney (GPHK) cell lines have been proven to be meaningful tools for the study on pathogenic infections in vitro. In this study, we aimed select most stable reference genes from seven housekeeping (Actin, B2M, GAPDH, RPL13, EF1A, 18S UBCE) applied two of (GPS GPHK) under both normal physiological conditions stimulated lipopolysaccharide (LPS) or polyinosi...

OBJECTIVES To develop a real-time quantitative RT-PCR method for BRCA1 mRNA and then use it for the study of BRCA1 gene expression in human MCF-7 breast cancer cells after their exposure to antineoplastic agents and gamma irradiation. DESIGN AND METHODS The developed QRT-PCR method is based on the real-time monitoring of a fluorescein-labeled TaqMan probe, specific for BRCA1 mRNA, during PCR ...

MicroRNAs (miRNAs) are small (approximately 22 nt) RNAs that play important roles in gene regulatory networks by binding to and repressing the activity of specific target mRNAs. Recent studies have indicated that miRNAs circulate in a stable, cell-free form in the bloodstream and that the abundance of specific miRNAs in plasma or serum can serve as biomarkers of cancer and other diseases. Measu...