The assessment of RNA integrity is a critical first step in obtaining meaningful gene expression data. Working with low-quality RNA may strongly compromise the experimental results of downstream applications which are often labour-intensive, time-consuming, and highly expensive. Using intact RNA is a key element for the successful application of modern molecular biological methods, like qRT-PCR...

Purpose: To investigate the mechanism of microRNA-124 action on neuroblastoma apoptosis induced by sevoflurane.
 Methods: MiR-124 expression was assessed in a cell line (SMS-KAN) using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The role miR-124 sevoflurane anesthesia-induced studied activity and analysis 3-(4, 5-dimethylthiazolyl-2-yl)-2-5 diphenyl tetrazolium ...

Macrophages can change their phenotypes according to the changes in microenvironment, and thus have various functions, that is, macrophages polarization. Macrophage phenotype is associated with progression of atherosclerotic plaques. Studies shown a large accumulation p62 protein Whether affects level macrophage polarization inflammation its mechanism not clear. The levels treated ox-LDL were d...

A quantitative, real-time PCR method was developed to enumerate Lactobacillus plantarum IWBT B 188 during the malolactic fermentation (MLF) in Grauburgunder wine. The qRT-PCR was strain-specific, as it was based on primers targeting a plasmid DNA sequence, or it was L. plantarum-specific, as it targeted a chromosomally located plantaricin gene sequence. Two 50 l wine fermentations were prepared...