vaccines is considered as a critical point and in-process of quality control (IPQC) test of vaccine production. Rapid tests, like real time PCR, are more appropriated when the production occurs at industrial scale because of the amounts of starting materials and the excess of consumed time required. In the current study, a real-time quantitative reverse transcription-polymerase chain reaction...

BACKGROUND Management of endometrial carcinoma (EC) still needs improvement of risk assessment. Recently, L1CAM immunohistochemical (IHC) evaluation showed a unique value to predict the outcome of early EC. However IHC results are often conflicting for lack of inter-laboratory standardisation. METHODS Here, as a proof of concept and to increase reproducibility we assayed eighty-two EC and 26 ...

Quantitative RT-PCR (reverse transcription polymerase chain reaction, also known as qRT-PCR or real-time RT-PCR) has been used in large proportions of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization using stably expressed genes, known as references. Statistical algorithms have been developed rece...

BACKGROUND Model-based analysis of data from quantitative reverse-transcription PCR (qRT-PCR) is potentially more powerful and versatile than traditional methods. Yet existing model-based approaches cannot properly deal with the higher sampling variances associated with low-abundant targets, nor do they provide a natural way to incorporate assumptions about the stability of control genes direct...

Abstract Background Circular RNAs (circRNAs) have been reported to be important regulators of the development and progression various carcinomas. However, role circRNA_100290 in gastric cancer (GC) is still unclear. This study aimed investigate GC invasion metastasis possible underlying mechanism. Methods The expression cells tissues was examined using quantitative real-time polymerase chain re...

A Imunodeficiência combinada grave (SCID) é uma doença primária letal, quando não empregado tratamento precoce, diagnóstico por meio da análise do círculo de excisão receptor células T (TREC) e o recombinação kappa (KREC). Por intermédio dos cartões Guthrie papel filtro, são extraídas as informações baseadas no teste molecular reação em cadeia polimerase (polymerase chain reaction, PCR), tempo ...

The clinical significance of human coronaviruses in more severe respiratory illnesses has recently been shown to be higher than was previously assumed. Rapid and reliable diagnosis of human coronavirus infections therefore becomes indispensable in a routine clinical setting. In this study, we present a very sensitive and specific TaqMan-based, real-time quantitative reverse transcriptase PCR (q...

Background. Nasopharyngeal swab/oropharyngeal swab (NPS/OPS) qRT-PCR is the gold standard for detecting SARS-CoV-2. However, it has its own limitations including cost and invasiveness. As an alternative, individual testing of saliva samples was validated shown to be comparable in sensitivity specificity with NP-OP qRT-PCR. To further maximize utility, researchers wish explore antigen pooled met...