background: candida albicans (c. albicans) is a major cause of candidaemia in people with impaired immunity. blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. we established a real-time pcr assay for c. albicans detection in blood by lightcycler pcr and melting curve analysis. methods: five milliliter blood samples from healthy volunt...

background : enteroaggregative e. coli (eaec) is increasingly recognized as a cause of often persistent diarrhea in children and adults in both developing and developed countries. the aim of the present study was to investigate the presence and the frequency of eaec as etiologic agent of diarrhea in shiraz. methods : a total of 715 stool samples were collected from patients with diarrhea in shi...

abstract        background and objective: herpes simplex encephalitis is a life-threatening consequence of the central nervous system (cns) infection with herpes simplex virus (hsv). although it is a rare disease, mortality rates reach 70% in the absence of therapy and only a minority of individuals can return to normal function. the aim of this study was to determine possible correlation betwe...

background and objectives: pseudomonas aeruginosa (pa) is one of the most important causes of nosocomial infections and has an intrinsic resistance to many antibiotics. among all the resistance-nodulation-division (rnd) pumps of p. aeruginosa, mexab-oprm is the first efflux pump found to target multiple classes of antibiotics. this study was aimed to evaluate the expression level of genes expre...

background and objectives: a new real- time pcr protocol for the detection of trichomonas vaginalis in pooled urine samples has been optimized and validated. materials and methods: the amplification protocol, targeting a 2kb repeated gene in the t. vaginalis genome, was optimized by varying pcr parameters. as a reference method, a real-time pcr protocol targeting the beta-tubulin gene (y. versl...