Phenoxazinone synthase, an oligomeric multicopper oxidase produced by Streptomyces antibioticus, is responsible for the six-electron oxidative coupling of two molecules of 4-methyl 3-hydroxyanthraniloyl pentapeptide to form the phenoxazinone chromophore of the antineoplastic agent actinomycin D. Spectroscopic studies have shown that the enzyme contains one type I (blue) and three to four type I...

In this paper we determine the local and global resilience of random graphs Gn,p (p n−1) with respect to the property of containing a cycle of length at least (1 − α)n. Roughly speaking, given α > 0, we determine the smallest rg(G,α) with the property that almost surely every subgraph of G = Gn,p having more than rg(G,α)|E(G)| edges contains a cycle of length at least (1−α)n (global resilience)...

Many fluids of hydrocarbon derivatives were used as working fluids in refrigeration and air conditioning during the past few decades. The phase out of chlorofluorocarbons and hydrochlorofluorocarbons has initiated research on finding an alternative working fluid for the existing. In this article, some working fluids with zero ozone depletion potential have been assessed for their suitability as...

Selenourea, SeC(NH2)2, recently found an application as a derivatization reagent providing a significant anomalous diffraction signal used for phasing macromolecular crystal structures. The crystal structure of selenourea itself was solved about 50 years ago, from data recorded on films and evaluated by eye and refined to R = 0.15 with errors of bond lengths and angles about 0.1 Å and 6°. In th...

In this paper analytical study has been made to investigate a hybrid refrigerant consists of different refrigerants with mixing ratios and find out its effects on the performance cycle specify best that gives least environmental pollution. Firstly vapor compression refrigeration system analyzed several pure refrigerants( R11, R12, R13,R22,R23, R32, R41, R115, R116, R124, R125, R134a, R142a, R14...

In the 90s, as in other countries, transformation of Chilean viticulture brought about introduction and spread European grapevine varieties which has resulted a massive loss minor local autochthonous traditionally grown several wine growing regions. Fortunately, recent years, minority have been revalued due to their high tolerance pests diseases because adaptation thermal water stress triggered...

Enerji verimliliği, küresel ısınma ve iklim değişikliği günümüzde üzerinde durulan en önemli konuların başında gelmektedir. Tüketilen enerjinin etkin verimli kullanımına ilişkin yeni tasarımlar yaparken de tasarımlarda kullanılacak yenilikçi teknolojiler ekonomi çevresel olumsuz etkilerin azaltılmasına katkı sağlayacaktır. Bu çalışmada; tamamen gaz yakıcı bir cihazla çalışan döküm sektöründe ku...

A Singular value decomposition (SVD)-enhanced Least-square fitting technique is discussed. By automatic identifying, ordering, and selecting dominant SVD modes of the derivative matrix that responds to the variations of the variables, the converging process of the Least Square fitting is significantly enhanced. Thus the fitting speed can be fast enough for a fairly large system. This technique ...

Wild-type and site-specific mutants C166S and C166A (Cys-166-->Ser and Cys-166-->Ala respectively) of the amidase (acylamide amidohydrolase, EC 3.5.1.4) from Pseudomonas aeruginosa were expressed in Escherichia coli by using the vector pKK223-3. Both mutant proteins were catalytically inactive but showed complete cross-reactivity with polyclonal antiserum raised against the wild-type enzyme, as...