The kinetics of oxidation of rainbow trout (Oncorhynchus mykiss) NADPH-cytochrome P450 reductase (NCPR) by cytochrome-c and NADH-ferricyanide reductase (NFR) by K3Fe(CN)6 were studied. NCPR and NFR activities from liver microsomes of rainbow trout (Oncorhynchus mykiss) had a specific activity of 0.0246 and 0.0350 nmoles/min/mg protein, respectively. The maximal rate of NCPR reaction was found a...

Myxobolus squamalis is a myxozoan skin parasite first reported from rainbow trout (Oncorhynchus mykiss). Identification of the parasite based on myxospore morphology is unreliable because M. squamalis is similar to several other myxobolids that share host species and geographic ranges. The only ssrRNA gene sequence available for M. squamalis is from Chinook salmon, Oncorhynchus tschawytscha, bu...

approved:__________________________________________________ Carl B. Schreck The extent to which stressful events are maladaptive or adaptive to the long-term survival of fish remains to be better understood. The aim of this study was to identify differentially expressed genes in the livers of rainbow trout, Oncorhynchus mykiss, responding to an experimental stressor. Gene expression responses w...

Estrogen receptor-mediated induction of zona radiata (ZR) and vitellogenin (VTG) mRNA and protein in rainbow trout (Oncorhynchus mykiss) was compared to assess their utility as biomarkers for exposure to estrogenic compounds. Partial sequences of rainbow trout ZR and beta-actin were cloned by reverse transcriptase polymerase chain reaction (RT-PCR) using degenerate primers based on conserved re...

Polymorphism. A variable number of tandem repeat (VNTR; Nakamura et al., 1987) polymorphism was detected in rainbow trout (Oncorhynchus mykiss Walbaum) using MspI and BgZII. The probe, RVF9303, was isolated from a genomic DNA cosmid library developed from the RTG-2 cell line (Wolf and Quimby, 1962). Method of Detection. Nylon filters containing rainbow trout genomic DNA digested with MspI o...

Spawning time in salmonids is a sex-limited quantitative trait that can be modified by selection. In rainbow trout (Oncorhynchus mykiss), various quantitative trait loci (QTL) that affect the expression of this trait have been discovered. In this study, we describe four microsatellite loci associated with two possible spawning time QTL regions in coho salmon (Oncorhynchus kisutch). The four loc...

Rainbow trout (Oncorhynchus mykiss) were exposed to 1.5 and 15% v/v secondary treated sewage effluent for 32 weeks in flow-through mesocosms. The exposure encompassed the full period of reproductive development for rainbow trout. Trout did not show any evidence of a dose-dependent change in growth. Fish exposed to 15% effluent were the only group to show mortality (5%) over the duration of the ...

The widespread use of plastic products in our modern life represents a serious threat to aquatic environments and wild animals that are exposed waste. Although microplastics (MPs) have been reported fish from several freshwater around the world, mountain little studied so far. occurrence MPs was assessed gastrointestinal tracts (GITs) non-native (rainbow trout Oncorhynchus mykiss brown Salmo tr...

Fish parvalbumin (PRVB) is an abundant and stable protein in fish meat. The variation in cross-reactivity among individuals is well known and explained by a broad repertoire of molecular forms and differences between IgE-binding epitopes in fish species. PVRB has "sequential" epitopes, which retain their IgE-binding capacity and allergenicity also after heating and digestion using proteolytic e...