In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current an...

A partial linkage map was constructed for the sweet cherry (Prunus avium L.) cultivar Emperor Francis from a population of 56 microspore-derived callus culture individuals. The callus cultures were genotyped for two allozymes and 90 random amplified polymorphic DNA (RAPD) markers using 79 random decanucleotide DNA primers and the polymerase chain reaction (PCR). Eighty-nine markers mapped to 10...

genetic diversity relationships of 50 isolates of cytospora schulzeri on apple from different parts of the semirom region were analyzed using 15 polymerase chain reaction (pcr) based markers, 7 random amplified polymorphic dnas (rapds) and 8 microsatellite primed polymerase chain reaction (mp-pcr). using 7 selected rapd primers 113 bands were generated, of which 81 bands were polymorphic (71.7%...

Adult specimens of the two common freshwater bivalves, Caelatura (Caelatura) companyoi and Caelatura (Caelatura) prasidens belonging to family Unionidae were collected from the River Nile at Giza governorate in Egypt. These two species were described by some authors as one species and others separated them as two different species. In the present study, random amplified polymorphic DNA-polymera...

Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers sh...

Understanding genetic structure and status of genetic variation of Fasciola hepatica isolates from different hosts, has important implications on epidemiology and effective control of fasciolosis. Random amplified polymorphic DNA (RAPD-PCR) was used to study the genetic variation of F. hepatica in sheep and cattle. DNA was extracted from adult helminthes removed from livers of each infected ani...

Fusarium verticillioides (F. verticillioides) is not only a primary pathogen of maize, but also can causedisease in other crops such as sorghum. Pathogenicity is related to mycotoxin production such as fumonisin.In the present study, 24 isolates of F. verticillioides, which were previously identified by phenotype basedmethods, were re-identified using restriction fragment length polymorphism (R...

In this study, we evaluated three PCR-based methods for the molecular typing of nonpathogenic Fusarium oxysporum isolates: random amplified polymorphic DNA (RAPD), polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and amplified fragment length polymorphism (AFLP). The analyses were performed using 64 isolates of F. oxysporum collected from cotton-producing areas in E...

Molecular genetics, and in particular the development of the polymerase chain reaction (PCR), has provided a way to distinguish these organisms. One primer pair specific for the genus Giardia is available (Mahbubani et al. 1991) as is a primer pair specific for G.intestinalis, (Mahbubani et al. 1992). During experiments in this laboratory, designed to compare strains and species of Giardia usin...

anopheles fluviatilis complex is known to be a vector of malaria in iran. since mosquitoes of this species cover a wide geographical range in iran, they might have evolved into different separated populations. random amplified polymorphic dna polymerase chain reaction (rapd-pcr) assay was used to differentiate geographic populations of this species. dna was extracted from individual mosquitoes ...