Microsatellites, tandem repeats of short nucleotide (1-6 bp) sequences, are the DNA marker of choice because of their highly polymorphic, ubiquitous distribution within genome, ease of genotyping through polymerase chain reaction (PCR), selectively neutral, co-dominant and multi allelic nature. Six microsatellites, three polymorphic and three monomorphic, have been characterized for the first t...

Information on the genetic structure of fish is a useful means for optimizing identification stocks, stock enhancement, breeding programs, management sustainable yield and preservation diversity. The population African Catfish, Clarias gariepinus (Burchell, 1822) in cultured feral populations from Rivers Guzuguzu, Fada Magaga (Kano State) were investigated using RAPD (Random Amplified Polymorph...

the last two decades have seen a massive growth in the application of dna technology in histocompatibility and immunogenetics. this review summarises the history and application of dna methods in this area.

Cronobacter genus bacteria are food-borne pathogens. Foods contaminated with spp. may pose a risk to infants or immunocompromised adults. The aim of this study was determine the microbiological quality nuts, seeds and dried fruits special emphasis on occurrence Analyses were carried out 64 samples commercial nuts (20 samples), (24), candied (8), (4), mixes seeds, (8). tested for total plate cou...

The fungus Cercospora beticola Sacc. is the one of the most important pathogens on the sugar beet. This plant pathogen causes cercospora leaf spot disease which is a serious economic problem for growers of this crop all over the world. Traditionally, the identification of C. beticola is based mainly on conidial characters and host association, but today molecular characterization is necessary f...

We describe polymerase chain reaction primers and amplification conditions for 13 highly polymorphic microsatellite DNA loci isolated from the Dalmatian wall lizard, Podarcis melisellensis. The number of alleles per locus ranged from 12 to 41, with levels of observed heterozygosity between 0.62 and 0.94. Most of these loci were successfully cross-amplified in the closely related species P. sicu...

Many reports highlight biological responses of crop plants after non-ionizing electromagnetic radiation (EMR) exposure based on the phenotypic and physiological levels. So, this study aimed to estimate genetic alterations in proteins, isozymes, DNA banding patterns as well extent nuclear damage economic corn (Zea mays) under stress EMR using accurate reliable bioassays like sodium dodecyl sulfa...

Samples of Echinococcus granulosus from seven pigs from Mexico were compared with isolates of the parasite from pigs in Poland and representative strains and species of Echinococcus. Isolates from pigs in Mexico were found to be genetically identical to E. granulosus from Polish pigs and distinct from other major genotypes by sequencing part of the mitochondrial cytochrome c oxidase I (COI) mtD...