DNA barcoding is used as a universal tool for delimiting species boundaries in taxonomically challenging groups, with different plastid and nuclear regions (rbcL, matK, ITS and psbA-trnH) being recommended as primary DNA barcodes for plants. We evaluated the feasibility of using these regions in the species-rich genus Terminalia, which exhibits various overlapping morphotypes with pantropical d...

We present the first national DNA barcode resource that covers the native flowering plants and conifers for the nation of Wales (1143 species). Using the plant DNA barcode markers rbcL and matK, we have assembled 97.7% coverage for rbcL, 90.2% for matK, and a dual-locus barcode for 89.7% of the native Welsh flora. We have sampled multiple individuals for each species, resulting in 3304 rbcL and...

The production of (82)Sr at iThemba LABS is performed by the proton bombardment of a RbCl target using the facility's Vertical-Beam Target Station (VBTS). (82)Sr is separated from the target material using a method based on target dissolution, using dilute ammonium chloride solution, and the use of chromatographic methods on Purolite S950 ion exchange resin. After performing a further purificat...

We examined three parallel data sets with respect to qualities relevant to phylogenetic analysis of 20 exemplar monocotyledons and related dicotyledons. The three data sets represent restriction-site variation in the inverted repeat region of the chloroplast genome, and nucleotide sequence variation in the chloroplast-encoded gene rbcL and in the mitochondrion-encoded gene atpA, the latter of w...

The morphologically variable moss Trichostomum brachydontium is very common in south and west Europe, particularly under Mediterranean Atlantic climates. A morphological study was conducted alongside a molecular phylogenetic based on nr ITS cp-rbcL regions order to assess if T. an exceptionally polymorphic species as evidenced by the number of described infraspecific taxa last century or, alter...