The Abbott RealTime HCV Genotype II kit is designed for use in the transcription and amplification of the 5’UTR or NS5B regions of the hepatitis C virus (HCV) genome. RealTime-PCR amplification is conducted in three separate reaction mixtures with different fluorophores where genotypespecific probes are used to detect HCV genotypes 1, 1a, 1b, 2, 3, 4, 5 and 6 such that: • Reaction A detects all...

Methods Sixty lymph node aspirates from suspected cases of tuberculous lymphadenitis were examined for Mycobacterium tuberculosis by Ziehl neelsen (ZN) staining, culture on a Lowenstein Jensen medium, and cytological examination for findings suggestive of tubercular lymphadenitis. DNA from all the samples was amplified by conventional PCR targeting 123 bp and 240 bp fragments of IS6110 and MPT6...

Ứng dụng kỹ thuật y sinh học phân tử trong xác định đột biến gen EGFR đã đem lại những lợi ích nổi bật điều trị đích bệnh nhân ung thư phổi không tế bào nhỏ (UTPKTBN), đó giải trình tự và Realtime PCR đang được sử phổ ở Việt Nam, cả 2 đều có ưu, nhược điểm riêng, thể phối hợp, hỗ trợ cho nhau. Nghiên cứu này thực hiện với mục tiêu: 1) Xác bằng trên mẫu mô UTPKTBN; 2) So sánh kết quả giữa PCR. T...

Bovine respiratory syncytial virus (BRSV) is a pneumovirus in the family paramyxoviridae, is an important cause of acute respiratory disease in postweaning calves and feedlot cattle. The real-time reverse transcriptase PCR protocols were developed to detect BRSV infection in infected animals. The sensitivity of RT-PCR protocols based on fusion gene were evaluated using different Mastermixes suc...

The real-time polymerase chain reaction (PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the DNA amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect amplification products. Appropriate data analysis and/or use of apposite chemistries ...