هدف: هدف پژوهش حاضر، ساخت و اعتبار­سنجی سیاهه آسیب‌شناسی روانشناختی نوجوانان در فضای مجازی بود. روش: روش آمیخته اکتشافی (کیفی-کمی) بدین منظور با استفاده از تحلیل مضمون مقالات مصاحبه‌های انجام‌شده متخصصان فعال مجازی، آسیب­ ها شناسایی شدند بر اساس آن ­ای ساخته شد. جامعه آماری بخش کیفی شامل مقالات، اساتید دانشگاه­ های اراک، اصفهان، امام خمینی قزوین شهر اراک بودند که نمونه هدفمند آنها انتخاب کمی بو...

BACKGROUND We compared the AdvanSure hepatitis B virus real-time polymerase chain reaction (AdvanSure HBV) kit with three other HBV DNA quantification assays and evaluated its performance. METHODS The AdvanSure HBV real-time PCR assay was compared with the Abbott RealTime HBV Quantification Kit, the COBAS TaqMan HBV Test, and the VERSANT HBV branched DNA 3.0 assay. The precision, linearity, a...

Traditionally, scientists and clinicians have used assays that involve growing cultures to screen samples for the presence of pathogenic microorganisms. Today, culture-based methods for pathogen detection are rapidly being replaced by faster and more specific Realtime PCR assays that discriminate between microorganisms based on a signal from specific nucleic acid sequences. Applied Biosystems (...

The management of invasive fungal infections has been hampered by the inability to make a diagnosis at an early stage of the disease. Molecular diagnosis by PCR appears very promising since fungal DNA can be detected in the blood of infected patients earlier than when using conventional methods. Recently, interest in the diagnosis of invasive fungal infections by real-time PCR has increased. Re...

References 1. Wilhelm J, Hahn M, Pingoud A. Influence of DNA target melting behavior on real-time PCR quantification. Clin Chem 2000;46:1738–43. 2. Zuna J, Muzikova K, Madzo J, Krejci O, Trka J. Temperature non-homogeneity in rapid airflowbased cycler significantly affects real-time PCR. Biotechniques 2002;33:508–12. 3. Schneider M, Joncourt F, Sanz J, von Kanel T, Gallati S. Detection of exon ...