objective(s): enterotoxigenic escherichia coli (etec) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. igy technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. the purpose of this study was to produce specific antibody by egg yolk antibody (igy...

background: rh (rhesus) is a highly complex blood group system in man deeply rooted in transfusion medicine. isolation of rhd from cord blod, cloning and expression of recombinant rhd antigen in bacterial expression system was the aim of this study. methods: total rnas were extracted from cord blood (o + ).  the quality of rna was determined by electrophoresis. in or­der to obtain coding sequen...

conclusions based on the findings, application of recombinant arcaga antigen for the detection of h. pylori infection is a simple, rapid, and brief non-invasive method. therefore, it can be suggested as an appropriate antigen for h. pylori detection kits. results we investigated caga protein, using the immunoblotting method in stool samples, which were already identified as positive by the appl...

background: echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus echinococcus, family taeniidae . we aimed to subclone antigen b gene in pqe-30 plasmid, its expression, and purification. methods: we subcloned hi gene into pqe-30 expression vector. the recombinant vector was transformed into e. coli , m15 and mass cult...

citrus tristeza virus (ctv) is among the most destructive pathogens of citrus and causes substantial economic losses in citrus-growing industry worldwide. considering recent distribution of this pathogen and its capability of transmission by existing aphid vectors in iran, detection of this virus is enforceable for controlling the damage caused by this pathogen in iran, as one of the major citr...

background and objectives: some of the human papillomaviruses (hpvs) can infect genital tracts and are sometimes associated with anogenital tract cancers. hpvs induced cervical cancers through the expression of e6 and e7 genes by inactivating the tumor suppressor proteins. in this study, e6 and e7 genes were chosen in order to construct an expression vector which is able to express target prote...

conclusions the r-b-flagellin carried antigenic epitopes just like the native flagellin, while the polyclonal antibody raised against it exhibited functional activity. results the polyclonal antibodies raised against this r-b-flagellin inhibited the motility of the homologous pao1 strain of p. aeruginosa, which significantly decreased the invasion of the pao1 strain into the a549 cells and also...

background: luteinizing hormone (lh) was secreted by the stimulating cells of the testes and ovaries in the anterior pituitary gland. the application of this hormone is in the treatment of men and women with infertility and amenorrhea respectively. m aterials and methods: in the present study the alpha and beta subunits of human lh gene were cloned into the pegfp-n1 expression vector and produc...

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...