objective(s):human cytomegalovirus (hcmv) remains a major morbidity and mortality cause in immuno suppressed patients. therefore, significant effort has been made towards the development of a vaccine. in this study, the expression of the pp65 and gb fusion peptides and fc domain of mouse igg2a as a novel delivery system for selective uptake of antigens by antigen-presenting cells (apcs) in pich...

the rotavirus nonstructural protein 4 (nsp4), is a multi functional protein that play key role in both viral morphogenesis and cytopathic effect associated with cell death. however, the complete biological effect of nsp4 remains to be clarified. since to obtain further knowledge about this protein there is a need for recognizing antibody and there is no commercial antibody against this protein...

interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (hcl). the aim of the present study is to apply yeast strain for secreting human ifnα2b fol...

objective: dna vaccines have been widely used to develop immunity against various pathogens including parasites and viruses. the potential of dna vaccine to induce an effective immune response is related to the expression levels of the encoded protein in eukaryotic cells. therefore, optimization of plasmid dna delivery system is a major concern in protein expression in order to make an efficien...

background: rfamide-related peptide-3 (rfrp-3) inhibits gonadotropin releasing hormone (gnrh) and luteinizing hormone (lh) secretion in rats. this study evaluates the effects of litter size and suckling intensity on rfrp mrna expression in the dorsomedial hypothalamic nucleus (dmh) of rats. materials and methods: a total of 32 pregnant and 4 non-lactating ovariectomized (control group) sprague-...

It is now 20 years since the tumor-associated factor parathyroid hormone-related peptide was identified. Since then, there have been significant changes in the understanding of the hypercalcemic syndromes associated with malignancy and with the role of this peptide in normal physiology as well as this specialized pathologic setting. Parathyroid hormone-related peptide has become a useful diagno...

Insulin release from the beta cells is influenced by nutrients (both carbohydrates and noncarbohydrates), hormonal factors including gut hormones and neural factors. Of all these insulin secretagogues, glucose availability is the major physiological determinant of insulin secretion. The insulin secretory response is greater after oral administration of glucose than after intravenous glucose adm...

The potential of “customizable units” to generate structural diversity for biological screenings is highlighted in this proof-of-concept synthesis new peptides related the potent antitumoral Sansalvamide A. Using L-4-hydroxyproline (Hyp) as a customizable unit linear parent peptide, an improved procedure selective peptide modification was developed. A divergent Hyp scission-reductive amination ...