HYPOTHESIS Treatment with anti-L-selectin monoclonal antibody will reduce venular neutrophil-endothelial rolling (flux and velocity) and adhesion associated with ischemia reperfusion injury in rat skeletal muscle. DESIGN Prospective, randomized experimental trials. SETTING Basic science research laboratory. MATERIALS Male Wistar rats weighing 109 +/- 5 g (mean +/- SEM). INTERVENTIONS Gr...

OBJECTIVES We sought to determine whether improving coupling between glucose oxidation and glycolysis by stimulating glucose oxidation during reperfusion enhances postischemic recovery of hypertrophied hearts. BACKGROUND Low rates of glucose oxidation and high glycolytic rates are associated with greater postischemic dysfunction of hypertrophied as compared with nonhypertrophied hearts. MET...

Introduction: Development of brain edema following focal cerebral ischemia exacerbates primary ischemic injury. Blood brain barrier (BBB) opening is an important part of edema named as vasogenic brain edema. In this study, quantitative alterations of BBB permeability is experimentally evaluated using transient focal cerebral ischemia in the rat. Methods: Two groups of male rats (ischemic and sh...

Introduction: It has been reported that traumas such as transverse abdominal incision before myocardial ischemia result in a significantly decreased infarct size. This phenomenon is named remote preconditioning of trauma. Since small skin burn is one of most common traumas, the effect of this injury on ischemia-induced arrhythmias and infarct size was investigated in a rat model of ischemia-...

OBJECTIVES Numerous mechanisms have been proposed to participate in adaptation of heart to ischaemia by ischaemic preconditioning. We have described previously a release of cardio-protective protein fraction during ischaemic preconditioning of dog heart. In the current study the effect of high soluble protein fraction (HS fraction) released from isolated perfused rat liver after ischaemia and r...

1. The aim of this study was to assess whether the protective effect of ischaemic preconditioning on endothelial function in coronary arteries of the rat involves kinins. 2. Isolated hearts of the rat were exposed to a 30-min low-flow ischaemia (flow rate of 1 ml min[-1]) followed by 20-min reperfusion, after which coronaries were precontracted with 0.1 microM U-46619, and the response to the e...

To examine the relevance of angiotensin II type 1 receptor (AT1R) expression in the determination of myocardial function after ischemia/reperfusion, Sprague-Dawley rats were treated intravenously with antisense oligodeoxynucleotides (AS-ODNs) directed at AT1R mRNA (100 microg/rat, n=9) or scrambled antisense oligodeoxynucleotides (Scr-ODNs, 100 microg/rat, n=6). Both AS-ODNs and Scr-ODNs were g...

BACKGROUND Ischemia and subsequent reperfusion induce lipid peroxidation in the cerebrum of the fetal rat. The present study evaluated the antioxidant activity of endogenous coenzyme Q in protecting against the lipid peroxidation induced in the fetal rat brain by ischemia/reperfusion. METHODS We used wistar rats at day 19 of pregnancy. Fetal ischemia was induced by bilateral occlusion of the ...

Heat-sensitive moxibustion has neuroprotective effects against focal cerebral ischemia/reperfusion injury, however its mechanism of action remains unclear. In this study, rat models of focal cerebral ischemia/reperfusion injury were treated with suspended moxibustion at acupoint Dazhui (DU14) for 35 minutes. Results showed that suspended moxibustion decreased infarct volume, reduced cortical my...

Wang, Lianguo, Gennady Cherednichenko, Lisa Hernandez, Jessica Halow, S. Albert Camacho, Vincent Figueredo, and Saul Schaefer. Preconditioning limits mitochondrial Ca21 during ischemia in rat hearts: role of KATP channels. Am J Physiol Heart Circ Physiol 280: H2321–H2328, 2001.—Prolonged myocardial ischemia results in an increase in intracellular calcium concentration ([Ca]i), which is thought ...