pathogenicity and genetic variability of 13 fusarium oxysporum isolates collected from sugar beet field of six provinces of iran during 1999- 2008 were evaluated. pathogenic variability of the isolates was determined using a root-dip assay in spore suspension in test tubes for four- week plants. the isolates were classified into strong and weak pathogenic groups. a total of 15 rapd primers were...

b ackground: the aim of the present study was to determine the molecular characteristics of echinococcus granulosus from paraffin-embedded tissues of hydatid cysts isolated from human and protoscoleces of hydatid cysts from sheep, cattle and camel isolates using pcr- rflp of its1- rdna analysis in golestan province, northern iran. m ethods: e . granulosus isolates from human patients infected w...

brucella transmission and epidemiology depend on infecting species and biovar. therefore, exact identification of the brucella is important to design correct control and treatment strategies. in this study, we examined presence of other brucellae in isfahan. one hundred twenty brucella isolates were collected and genomic dna was extracted from them. omp2a fragment of all isolates were amplified...

In a tuberculosis (TB) program in the Central Penitentiary Hospital of Azerbaijan, we analyzed 65 isolates of Mycobacterium tuberculosis by IS6110-based restriction fragment-length polymorphism (RFLP) and spoligotyping. From 11 clusters associated with 33 patients, 31 isolates had an IS6110-based banding pattern characteristic of the Beijing genotype of M. tuberculosis. In addition, 15 M. tuber...

Giardia duodenalis causes diarrhoea and malabsorption. The objectives of the study were to detect local isolates of G. doudenalis by polymerase chain reaction (PCR) and to determine their restriction fragment length polymorphisms (RFLP). G. doudenalis isolated from stools of patients from Hospital Orang Asli Gombak were cultured axenically using TYI-S-33 medium with 10% foetal calf serum. The c...

in order to study the genetic diversity of the causal agent of fusarium wilting in chickpea, two techniques including rapd and pcr-rflp were used. in rapd-pcr technique, all 14 primers could show the diversity among the isolates. after studding the banding patterns of 14 primers, a dendrogram based on similarity matrix was constructed. the rapd dendrogram analysis could not separate isolates ba...

Beauveria spp. (one Beauveria sp., two B. brongniartii, and 47 B. bassiana) isolated from insects and soil from Kenya and other 16 different countries, were obtained from the CABI Bioscience Genetic Resource Collection. DNA was extracted from the 50 isolates and their genetic variability was investigated using restriction analysis of the internal-transcribed-spacer ribosomal region restriction ...

BACKGROUND The aim of the present study was to determine the molecular characteristics of Echinococcus granulosus from paraffin-embedded tissues of hydatid cysts isolated from human and protoscoleces of hydatid cysts from sheep, cattle and camel isolates using PCR- RFLP of ITS1- rDNA analysis in Golestan Province, northern Iran. METHODS E. granulosus isolates from human patients infected with...