objective: toxoplasmosis can lead to severe pathological effects in both infected humans and animals. the various dna vaccines against toxoplasma compose of single or cocktail antigens have been investigated but they have partial protective against disease. in this study, we used pcrop1 as a dna vaccine and aluminium phosphate and aluminium hydroxide to compare their efficacy as mineral adjuvan...

toxoplasma gondii contain various immunogenic antigens. the most important toxoplasma antigens are somatic and excreted/secreted antigens. rhoptry proteins are known as excreted/secreted antigens. these antigens have been proposed as a vaccine candidate against toxoplasmosis. the main objective of the present work was cloning rhoptry protein1 (rop1) gene of toxoplasma gondii (rh) in a cloning v...

Toxoplasma gondii is the causative agent for toxoplasmosis. The rhoptry protein 1 (ROP1) is secreted by rhoptry, an apical secretory organelle of the parasite. ROP1 plays an important role in host cell invasion. In this study, the efficacy of ROP1 as a vaccine candidate against toxoplasmosis was evaluated through intramuscular or subcutaneous injection of BALB/c mice followed by immunological c...

  Toxoplasma gondii contain various immunogenic antigens. The most important Toxoplasma antigens are somatic and excreted/secreted antigens. Rhoptry proteins are known as excreted/secreted antigens. These antigens have been proposed as a vaccine candidate against toxoplasmosis. The main objective of the present work was cloning rhoptry protein1 (ROP1) Gene of Toxoplasma gondii (RH) in a cloning...

background toxoplasma gondii is an obligatory intracellular protozoan parasite, which infects human beings. since the current antigens used for diagnosis or vaccination are contaminated with non parasitic material in which the parasite is grown, it is tried to produce recombinant antigens to design vaccines against toxoplasmosis, or make diagnostic kits. choosing the type of antigen to produce ...

BACKGROUND Toxoplasmosis is a worldwide-distributed infection which is mostly asymptomatic but can cause serious health problems in congenitally-infected newborns and immunecompromised individuals. Research is undergoing both to improve Toxoplasma serological tests, which play the main role in laboratory diagnosis of the infection, and develop an effective vaccine to prevent the infection. Some...

A goal of the current study was to evaluate serological applications of Toxoplasma gondii GRA2 and rhoptry protein 1 (ROP1) antigens. Soluble recombinant GRA2 and ROP1 antigens as fusion proteins containing six histidyl residues at the N and C terminals were obtained using an Escherichia coli expression system. Purification by one-step metal affinity chromatography allowed recovery of milligram...