The effect of interferon on the synthesis of the RNA species and proteins of vesicular stomatitis virus has been studied in two cell types. Virus protein synthesis is inhibited by interferon despite the apparent presence of near normal amounts of virus RNA with sedimentation values characteristic of virus messenger RNA. The synthesis of those virus RNA species which are completely dependent on ...

A rat type C virus spontaneously activated from the NRK (normal rat kidney) cell line was found to have two major size classes of viral RNA subunits sedimenting at 35 and 30 S. Virus-producing cells contained both RNA species, while normal "virus-free" rat cells contained primarily virus-specific 30S RNA species. A DNA transcript, specific for Kirsten sarcoma virus, prepared from virus activate...

Nodamura virus, a small non-enveloped RNA virus, contains two species of RNA sedimenting at 22S (RNA-1) and 15S (RNA-2), a single major polypeptide of mol. wt. 40 X 10(3) and two minor polypeptides, of mol. wt. 38 and 43 X 10(3). Evidence is presented that the two RNA species are in the same particle. Although extraction of the virus with SDS-phenol yields the two species of RNA as separate ent...

A model of a multidomain complex is constructed using molecular modeling methods to explain the mechanism inhibitory effect favipiravir on RNA-dependent RNA polymerase (RdRp) SARS-CoV-2 coronavirus. As initial atomic coordinates, we use cryoelectron microscopy data for apo form RdRp virus and structure hepatitis C virus. After appropriate substitutions, an containing chains potential enzyme inh...

The Pichinde virus RNA did not possess the following characteristics of eucaryotic mRNA: polyadenylic acid sequence, capped methylated structure, and ability to direct protein synthesis in vitro. Polysomal RNA extracted from cells infected with Pichinde virus reannealed with 32P-labeled virus RNA, protecting about 60% of the latter against RNase degestion. The polyadenylic acid-containing polys...

Narnavirus 23S RNA is a persistent positive-stranded RNA virus found in yeast Saccharomyces cerevisiae. The viral genome (2.9 kb) only encodes its RNA-dependent RNA polymerase, p104. Here we report the generation of 23S RNA virus, with high frequency, from a vector containing the entire viral cDNA sequence. When the conserved GDD (Gly-Asp-Asp) motif of RNA-dependent RNA polymerase was modified,...

Background and Aims: Influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. A variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. Methods: Nasal and pharyngeal samples taken from patients were inoculated into Madin-Darby canine kidney (MOCK) cells and embryonated chicken eggs (ECEs). The culture media was a...

A ribonucleic acid (RNA)-dependent RNA polymerase was induced in chick embryo fibroblast cells after infection with Sendai virus (parainfluenza 1 virus). The enzyme was associated with the microsomal fraction of infected cells and reached maximum detectable activity at 18 hr after virus infection. The activity of the enzyme in vitro was dependent on the presence of added magnesium ions and all ...