to detect hybrid particles. Although the RNA of dependent virus often replicated to a level about 10 per cent of that of supporting virus,' maturation of intact dependent virus within heterologous capsid was much less efficient. This could be due to a better fit between capsomeres and homologous RNA or it could be due to a spatial relationship between capsomere protein synthesis and the viral R...

Spondweni virus (SPONV), a possible emerging virus, is member of the Flaviviridae family, genus Flavivirus and belongs to serogroup with Zika virus. The latest epidemic fever, which broke out in Western Hemisphere, warns about risk corresponding urban potential SPONV calls for design anti‑SPONV therapies. Previous studies have demonstrated that viral RNA helicases represent pr...

abstract : avian influenza virus (aiv) infection is a major cause of influenza mortality in birds and can cause human mortality and morbidity. although the risk of infection with avian influenza virus (aiv) is generally low for most people, the pathogenic virus can cross the species barrier and acquires the ability to infect and be transmitted among the human population; therefore the rapid ide...

patients and methods five hundred and sixteen serum samples of the patients undergoing hemodialysis and kidney transplantation from various cities of khuzestan province were collected. anti-hepatitis g e2 antibodies were investigated by elisa method. rnas were extracted from serums and hepatitis g rna was detected by rt-pcr. results of the 516 samples, 38 (7.36%) specimens were positive for ant...

patients and methods 720 cases including 120 patients undergoing hemodialysis, and 600 patients with hiv infection were studied. all cases with positive results for hbsag were evaluated for the presence of anti-hdv antibodies. samples with anti-hdv positive results were subjected to nested pcr for hdv-rna confirmation, and sequenced for hdv genotype determination. results hbsag was found in 9 (...

The roles of the 3'- and 5'-terminal nucleotides and the panhandle structure of influenza B virus virion RNA were analyzed in vitro by transcription of model RNA templates with influenza B virus RNA polymerase. The results suggest that the stability of the panhandle and breathing of the extreme ends of the panhandle are important factors for efficient transcription. Influenza B virus polymerase...

background and aims: avian metapneumovirus (ampv) is rna virus responsible for upper respiratory disease in poultry which is usually accompanied by secondary bacterial infections. in iran, some molecular and serological studies indicated that ampv infection occurred in commercial poultry industry. materials and methods: in this study ampv was molecularly identified and characterized in an affec...

background and aims: virus-induced gene silencing (vigs) is a virus vector technology that exploits antiviral defense mechanism. by infecting plants with recombinant viruses containing host genes inserted in the viral genome, vigs achieves the rna silencing process. the purpose of this study was to investigate the possibility of tomato (lycopersicon esculentum mill.) and tobacco (nicotiana bent...

Mouse cells productively infected with Moloney murine leukemia virus were treated with interferon, and intracellular virus-specific RNA was studied by hybridization with complementary DNA. The steady-state concentration of virus-specific RNA in interferon-treated cells was somewhat greater than that in untreated cells, and the rates of virus-specific RNA synthesis were approximately equal in tr...

To study the effect of virus-specific antisense RNA expression on vesicular stomatitis virus (VSV) infectivity in cultured cells, a HeLaS3 cell line constitutively expressing antisense RNA complimentary to a portion of the VSV large RNA-dependent RNA polymerase gene (L) was established (HeAntiL). At an m.o.i. of 0.01 or 0.1, the HeAntiL cell line was able to reduce virus titre and delay virus-i...