In this study, the kinetic of biogas production was studied by performing a series laboratory experiment using rumen fluid of animal ruminant as inoculums. Cattle manure as substrate was inoculated by rumen fluid to the anaerobic biodigester. Laboratory experiments using 400 ml biodigester were performed in batch operation mode. Given 100 grams of fresh cattle manure was fed to each biodigester...

Chilling of whole rumen contents prior to preparation of strained rumen fluid enriched with particle-associated microorganisms resulted in rumen inoculum with the highest microbial dry weight, total bacterial counts, and degradation rates for casein and soybean meal. Strained rumen fluid, whole rumen contents blended with strained rumen fluid, chilled strained rumen fluid, and strained rumen fl...

A single injection of 15 g NaCl into the rumen, which increased the osmotic pressure of rumen fluid from 369 to 474 mOsmol/kg, resulted in a decrease in the transfer of blood urea into rumen fluid from 2.3 to 1.6 g N/day in sheep fed 50 g of chaffed oaten hay every hour. It is concluded that the reduced urea transfer was the result of a reduced urea input via saliva. The rumen wall remained vir...

It has been suggested that the rumen microbiome and rumen function might be disrupted if methane production in the rumen is decreased. Furthermore concerns have been voiced that geography and management might influence the underlying microbial population and hence the response of the rumen to mitigation strategies. Here we report the effect of the dietary additives: linseed oil and nitrate on m...

An artificial rumen for the study of nutrient digestion by the micro-organisms of the rumen was initially developed by Louw, Williams & Maynard (1949). In this, the micro-organisms were contained in a semi-permeable dialysis sac and the diffusible products of digestion removed by a salt solution, the composition of which approximated to that of saliva and which flowed continuously past the outs...

1. Three experiments were conducted to determine the flow of nitrogen through the rumen and abomasum when cows, steers and lambs were totally nourished on volatile fatty acids infused into the rumen. 2. In two dairy cows (650-700 kg) and two large steers (370-405 kg) the daily flow of non-ammonia-N (NAN) from the rumen was 50.7 and 58 mg/kg live weight (W)0.75 respectively. 3. The flows of NAN ...

Rumen is a persistent and specific ecosystem consists of bacteria, protozoa and fungus where feed fermentation takes place in it. Produced Hydrogen in rumen can be used in the synthesis of the volatile fatty acids and the microbial protein and its excess would be eliminated through the production of Methane by methanogenesis. Nutritionists have tried to find ways to decrease loss and energy and...

The major aim of this study was to compare the morphology and activity of rumen protozoa of Khuzestan water buffalo and Holstein cow using in vitro digestibility and gas production parameters of steam treated sugarcane pith. Rumen fluid obtained from two buffalo and cow steers fed the same diet, 30:70 concentrate: forage. To separate rumen protozoa, antibiotic solution and fungicides were added...

Anaerobic fungi inhabit the rumen and actively degrade plant cell walls. Rumen fungi produce high levels of cellulases and hemicellulases and are particularly proficient in producing xylanases. These enzymes are regulated by substrate (especially soluble sugars) available to the organisms. Fungi degrade unlignified (i.e., no histochemical reaction for phenolics) plant walls totally, indicating ...

1. Fistulated sheep given a concentrate diet were used to study the behaviour of solute ([51Cr]EDTA) and particle ([103Ru]phenanthroline) markers in the stomach under conditions of continuous feeding. 2. An injection of a mixed dose of [51Cr]EDTA and [103Ru]phenanthroline was given into the rumen and the time course of marker concentrations in the rumen and the abomasum was recorded. The curves...