background and objectives: streptococcus pneumoniae, a major human pathogen, is closely related to the commensal species s. mitis and s. oralis. s. pneumoniae surface proteins are implicated in virulence and host interaction of this species, but many of them have recently been detected in s. mitis b6 in silico. we tested for the presence of such genes usinga set of eight s. mitis and eleven s. ...

background: identifying regional types and evaluating the frequency of pneumococcal strains has become increasingly important especially in vaccination. the purpose of this study was the identification and frequency determination of our regional serotype and evaluation of the performance of recent type specific multiplex pcr for the diagnosis of streptococcus pneumonia serotypes. methods: all i...

results the number of s. pneumoniae isolates resistant to benzylpenicillin, imipenem, oxacillin and ceftazidime were 94.5%, 100%, 100%, and 21.8%, respectively. analysis of mutation in the genes for pbp showed that 85% of isolates had mutations in pbp2x, pbp2b and pbp1a. susceptibility to benzylpenicillin was decreased once the number of mutated pbp genes in s. pneumonia increased. according to...

results in total, 73 isolates were collected and diagnosed as s. pneumoniae. isolates were susceptible to ofloxacin 95.9%, vancomycin 93%, penicillin 78%, trimethoprim-sulfamethoxazole 61.6%, ceftriaxone 53.5%, meropenem 52%, cefotaxime 46.5%, and erythromycin 8.2%. of the 15 prsp isolates, the pbp2b gene was identified in 12 (80%). in 1 penicillin-intermediate s. pneumoniae isolate the pbp2b w...

background and objectives:   many surface proteins are implicated in nasopharyngeal colonization and pathogenesis of streptococcus pneumoniae . some of these factors are candidate antigens for protein based vaccines. new vaccine designs focus on the surface proteins (e. g., pspa and pspc ) and also cytolysin, and pneumolysin. in this study, 3 key virulence genes, lyta , pspc , and rrga , which ...

The aim of the present research was focused on the antibacterial, preliminary phytochemical and pharmacognostical properties of Vicoa indica (L.)DC. via in vitro approach. The aqueous and organic solvent (hexane, chloroform and methanol) extracts from the leaves of Vicoa indica (Asteraceae) were tested against Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Vibrio parahaemolyt...

Objective(s): Macrolide resistant Streptococcus pneumoniae pose an emerging problem globally. The aim of this study was to investigate the prevalence of ermB and mefA genes (macrolide resistant genes) by polymerase chain reaction (PCR) method and to detect drug resistance patterns of S. pneumoniae isolated from clinical samples to macrolides and other antibiotic agents by E-test method. Materia...

Background: Antibacterial proteins are widely expressed in snake venoms. Previously, we have isolated two immunodominant proteins with molecular weights of 14 and 65kD from the snake venom of Naja naja oxiana (N. oxiana). It was demonstrated that they had potent inhibitory effects against gram-positive bacteria, S. aureus and B. subtilis but were less effective against gram-negative bacteria, s...

introduction : streptococcus pneumoniae cause morbidity and mortality in infants and younger children. because of high prevalence of penicillin resistance, rapid and reliable diagnostic techniques for penicillin non-susceptible s. pneumoniae (pnssp) are important for prevention and treatment. we investigated the association of the restriction length polymorphism (rflp) patterns for pbp2b to dis...

Background: To date, more than 90 Streptococcus pneumoniae (S. pneumoniae) capsular serotypes are known. The prevalence of these serotypes varies according to the geographical area and the regional vaccination program. Due to the lack of regular vaccination programs for S. pneumoniae in developing countries, serotyping of the prevalent isolates is useful in selecting the correct vaccine. The pr...