In this rapid, simple enzyme immunoassay for carcinoembryonic antigen (CEA) in serum the "sandwich" system is used. The CEA is first extracted from the serum into acetate buffer by heating at 70 degrees C, then incubated in a polyvinyl microtitration plate previously coated with anti-CEA. The bound CEA is then reacted with anti-CEA conjugated to horseradish peroxidase and the activity of the pe...

The advanced Automated Fibre Placement (AFP) manufacturing technologies make the synthesis of Variable Angle Tow (VAT) composites, which enable design lightweight sandwich structures to possess variable stiffness facesheets. However, both global and local instability phenomena VAT plates under in-plane compressive loads are rarely explored till now. objective this article is fill gap by develop...

The question motivating the present study is whether metal sandwich plates with su3ciently strong cores are able to sustain substantially larger blast loads than monolithic solid plates of the same material and total mass. Circular plates clamped at their edges are considered under blast loads large enough to produce substantial de5ections. The material is elastic–perfectly plastic. Material st...

BACKGROUND: A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were collected by biotin-streptavidin affinity binding and detected by fluorescence signal generated by alkaline phosphatase. The 18S rRNA and SUC2 mRNA of Saccharomyces cerevisiae...