Central challenges in the design of large and dynamic macromolecular assemblies for synthetic biology lie in developing effective methods for testing design strategies and their outcomes, including comprehensive assessments of solution behavior. We created and validated an advanced design of a 600-kDa protein homododecamer that self-assembles into a symmetric tetrahedral cage. The monomeric uni...

Structural modifications of AOT/water/isooctane reverse micelles due to incorporation of proteins were studied at various water contents and protein concentrations, using small-angle X-ray scattering (SAXS) experiments under static conditions, rheometry analysis, and SR-SAXS experiments under induced shear flow. Two proteins, lysozyme (pI 11.1, Mw 14,300 Da) and BSA (pI 4.3, Mw 66,700 Da), were...

Dimyristoylphosphatidylglycerol (DMPG) has been extensively studied as a model for biological membranes, since phosphatidylglycerol is the most abundant anionic phospholipid in prokaryotic cells. At low ionic strengths, this lipid presents a peculiar thermal behavior, with two sharp changes in the light scattering profile, at temperatures named here T(on)(m) and T(off)(m). Structural changes in...

Introduction To date there are still many unknowns on how particles are formed from gas phase reactive systems such as combustion. It is possible to study particles in-situ using X-ray scattering techniques: Separate applications have shown that the size distribution of soot particle can be measured using Small-Angle X-ray Scattering (SAXS) [1-4] and particle structure with Wide-Angle X-ray Sca...

The SIBYLS beamline (12.3.1) of the Advanced Light Source at Lawrence Berkeley National Laboratory, supported by the US Department of Energy and the National Institutes of Health, is optimized for both small-angle X-ray scattering (SAXS) and macromolecular crystallography (MX), making it unique among the world's mostly SAXS or MX dedicated beamlines. Since SIBYLS was commissioned, assessments o...

High energetic ions can lead to the formation of long columnar defects along the ion trajectories, so called “ion tracks”. In amorphous metals, overlapping tracks produce macroscopic anisotropic growth phenomena [1]. Characterization and imaging of single ion tracks in these materials is challenging and has been successful only in a few cases using chemical etching [2], scanning probe [3] and t...

A set of LiFePO 4 samples has been studied by small-angle X-ray scattering (SAXS). Using the technique artificial neural networks, shape SAXS curves reconstructed taking into account hardware function. Estimates homogeneitiessizes and shapes in have obtained. Keywords: Small-angle scattering, network, lithium-ion rechargeable batteries, incorrect tasks regularization.

High pressure (HP) or urea is commonly used to disturb folding species. Pressure favors the reversible unfolding of proteins by causing changes in the volumetric properties of the protein-solvent system. However, no mechanistic model has fully elucidated the effects of urea on structure unfolding, even though protein-urea interactions are considered to be crucial. Here, we provide NMR spectrosc...

In this work we report the structural liposome modifications during DNA complexation using Small Angle X-ray Scattering (SAXS) technique. We obtained in situ complexation using an in line microchannel SAXS sample holder coupled to a fed-batch reactor and thus we were able to determine the electronic density profile and other liposome parameters in the presence of DNA.

Rhamnolipids (RL) are one of the most important classes of biosurfactants produced by microorganisms using a wide range of carbon sources, from a simple carbon source like glucose to complex wastes such as the used cooking oils used in this work. The objective of this work was to learn about the rhamnolipid-phospholipid dipalmitoyl phosphatidyl choline (DPPC) molecular interactions through the ...