We have used homology modelling, based on the crystal structure of the human glutathione S-transferase (GST) A1-1, to obtain the three-dimensional structures of rat GSTA3 and rat GSTA5 subunits bound to S-aflatoxinyl-glutathione. The resulting models highlight two residues, at positions 208 and 108, that could be important for determining, either directly or indirectly, substrate specificity fo...

BACKGROUND Patients with HBeAg-negative chronic hepatitis B (CHB) has a significantly different prognosis than inactive carriers; there is however, no reliable strategy for accurately differentiating these two disease conditions. OBJECTIVES To determine a strategy for discriminating patients with HBeAg-negative CHB from inactive carriers. MATERIALS AND METHODS Consecutive inactive carriers ...

BACKGROUND The incidence of acute coronary syndrome (ACS) in young people (≤65 years) is continuously rising. While prognostic factors in ACS are well-investigated less attention has been paid to their age-dependent prognostic value and their particular relevance in younger patients. The aim of our study was to assess the age-dependent prognostic impact of butyrylcholinesterase (BChE). METHOD...

Neuron membrane changes and ion redistribution during normoxic spreading depression (SD) induced, for example, by potassium injection, closely resemble those that occur during hypoxic SD-like depolarization (HSD) induced by oxygen withdrawal, but the degree to which the two phenomena are related is controversial. We used extracellular electrical recording and imaging of intrinsic optical signal...

* Corresponding author. Chairman of the Accreditation Assessment Committee, Department of Cardiology, Hammersmith Hospitals NHS Trust at Charing Cross Hospital, Fulham Palace Road, London W6 8RF, UK. Tel.: C44 208 846 1917; fax: C44 208 746 8182. E-mail address: [email protected] (K.F. Fox). 1 The Accreditation Assessment Committee of the European Association of Echocardiography. 2 President...

Caspase-3 is a critical effector caspase in apoptosis cascade, and is often over-expressed in many cancer tissues. The first synthesized procaspase-3 activator, PAC-1, induces cancer cell apoptosis and exhibits antitumour activity in murine xenograft models. To identify more potent procaspase-3 activators, a series of compounds were designed, synthesized and evaluated for their ability of induc...

Endoplasmic reticulum (ER) oxidoreductin 1α (Ero1α) is a disulfide producer in the ER of mammalian cells. Besides four catalytic cysteines (Cys(94), Cys(99), Cys(394), Cys(397)), Ero1α harbors four regulatory cysteines (Cys(104), Cys(131), Cys(208), Cys(241)). These cysteines mediate the formation of inhibitory intramolecular disulfide bonds, which adapt the activation state of the enzyme to th...

This paper presents a new combined symmetric key cryptographic technique, which is generally an amalgamation of Bit Manipulation, generalized Modified Vernam Cipher, Single Bit Manipulation and Modified Caesar Cipher. The technique proposed here is basically an advanced and upgraded module of SD-AREE cryptographic method, which is based on Modified Caesar Cipher along with Bit Manipulation and ...

1Department of Mathematics & Statistics, Indian Institute of Technology, Kanpur 208 016, India 2Department of Mathematics, The LNM Institute of Information Technology, Jaipur 302 031, India 3TIFR Centre For Applicable Mathematics, Tata Institute of Fundamental Research, Bangalore 560 065, India 4Department of Aerospace Engineering, Indian Institute of Technology, Kanpur 208 016, India *Correspo...