Snake venoms comprise a highly complex mixture of proteins, and there is also high interspecific intraspecific variability in their composition, even the same region. Our aim was to compare composition Bothrocophias myersi, Crotalus durissus Bothrops asper, snakes from Andean region Colombia by Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC). The were given research group under a...

The characterization of amyloid-beta peptide (Aβ) oligomer forms and structures is crucial to the advancement in the field of Alzheimer´s disease (AD). Here we report a critical evaluation of two methods used for this purpose, namely sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), extensively used in the field, and ion mobility coupled to electrospray ionization mass spect...

Two-dimensional gel electrophoresis (2-DE) is able to separate hundreds to thousands of proteins or polypeptides by coupling IsoElectric Focusing (IEF) in first dimension and Sodium Dodecyl Sulphate PolyAcrylamide-Gel Electrophoresis (SDS-PAGE) in second dimension. This particular configuration is called classical 2-DE: IEF separates proteins in function of their isoelectric point (pI) and SDS-...

This paper deals with three basic aspects: 1) basics of protein structure in relation to physicochemical characterization, protein stability, importance of disulfide bond and their structure; 2) basic principles involved in polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE and the rationale behind the use of disc electrophoresis; and 3) detailed protocol involved in SDS-PAGE in terms of re...

A quantitative urea-based amyloid b (Ab)-sodium dodecyl sulfate-polyacrylamide gel electrophoresis with Western immunoblot (Ab-SDS-PAGE/immunoblot) reveals highly conserved and disease-specific Ab peptide patterns (Ab 1-37, 1-38, 1-39, 1-40, 1-42) in Alzheimer’sdisease (AD)patientsandnondementedcontrols. For further standardization of this method, we analyzed cerebrospinal fluid (CSF) of eight ...

in the present study, we investigated total cell protein patterns of ten isolates of trichophyton rubrum by sds-page on 12.5% polyacrylamide resolving gels. twenty-two protein bands were detected with molecular weights in the range of 23.2 to 131.8 kd. proteins of 23.2, 25.4, 26.7, 28.2, 30.2, 33.1, 34.7, 38,41.7, 47.9, 52.5, 56.2, 61.7, 67.6, 70.8, 75.9, 80, 84.1, 93.3, 102.3, 114.8, 131.8 wer...