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Background: Dicrocoelium dendriticum, a liver trematode of herbivores, and man is very prevalent among ruminants of Iran, resulting in causing significant economic losses. Recently its phenotype polymorphism has been found in Iran, a phenomenon that may affect the protein profile of the parasite. Objectives: The aim of this study was to determine the somatic protein profiles of D.dendriticum in...
IB-16385A Abstract Capillary electrophoresis sodium dodecyl sulfate (CE-SDS), is the modern equivalent of the slabgel sizing technique SDS-PAGE. Common uses of SDS-PAGE include monitoring of manufacturing consistency and apparent molecular weight. Although used frequently, SDS-PAGE is a poor technique for quantitative protein purity due to inherent sample preparation artifacts, migration time a...
Cytosolic and membrane-associated proteins prepared from rice cells were separated and compared by two different 2D-PAGE methods, isoelectric focusing (IEF)/SDS-PAGE and nonequilibrium pH gradient electrophoresis (NEPHGE)/SDS-PAGE. Although IEF/SDS-PAGE of the cytosolic proteins showed sufficient resolution, some mitochondrial and basic microsomal membrane-associated proteins were weakly or har...
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