Complement-mediated release of enzyme molecules from reversed-phase evaporation vesicles serves as the basis of the sensitive homogeneous immunoassay reported here. We found it necessary to co-entrap the substrate glucose 6-phosphate with the bacterial enzyme glucose-6-phosphate dehydrogenase (EC 1.1.1.49) to protect enzyme activity during liposome preparation. Enzyme can be released specifical...

The single biggest challenge now facing drug delivery (for liposomes and indeed other carriers) is to initiate and produce release of the encapsulated drug only at the diseased site and at controllable rates. Our efforts have focused on developing a new thermal-sensitive drug delivery system, specifically for the local control of solid tumors. We describe here a new lipid formulation containing...

Research on liposome formulations has progressed from that on conventional vesicles to new generation liposomes, such as cationic liposomes, temperature sensitive liposomes, and virosomes, by modulating the formulation techniques and lipid composition. Many research papers focus on the correlation of blood circulation time and drug accumulation in target tissues with physicochemical properties ...

Hybrid liposome/metal nanoparticles are promising candidate materials for biomedical applications. However, the poor selectivity and low yield of the desired hybrid during synthesis pose a challenge. We designed a programmable liposome by selective encoding of a reducing agent, which allows self-crystallization of metal nanoparticles within the liposome to produce stable liposome/metal nanopart...

Tobacco mosaic virus (TMV) RNA was encapsulated in large, unilamellar phospholipid vesicles (liposomes), and the encapsulated TMV RNA was shown to be infectious when incubated with tobacco protoplasts under appropriate conditions. Maximal virus production in protoplasts was observed after their incubation with TMV RNA entrapped in phosphatidylserine/cholesterol liposomes. Infection was dependen...

Liposomes are spherically shaped phospholipid bilayers that used to encapsulate hydrophilic or lipophilic drugs. To have efficient drug delivery by a liposome, it should be able deliver the specific binding site, where can affect target. However, sometimes, due untimely release, this goal cannot achieved. Therefore, permeation rate of through liposomal membrane is important, as affects drug’s r...

The dynamics of liposome solubilization was monitored by dynamic light scattering and optical microscopy. A newly designed Y-shape microchannel connected to a room was incorporated into a microchip and the reaction processes of the liposome suspension and surfactant solution were observed in the room after mixing the two fluids and stopping the flow. By using this microchip, we succeeded in r...