Proton transport catalyzed by the nitrate-insensitive, vanadate-sensitive H(+)-ATPase in microsomes from maize (Zea mays L.) roots washed with 0.25 molar KI decreased as a function of time at 0 to 4 degrees C. The rate of proton transport was approximately one-half of that by freshly isolated microsomes after 6 to 18 hours of cold storage. The decrease in proton transport coincided with losses ...

Since the mechanism underlying the insulin stimulation of (Na+,K+)-ATPase transport activity observed in multiple tissues has remained undetermined, we have examined (Na+,K+)-ATPase transport activity (ouabain-sensitive 86Rb+ uptake) and Na+/H+ exchange transport (amiloride-sensitive 22Na+ influx) in differentiated BC3H-1 cultured myocytes as a model of insulin action in muscle. The active upta...

We study charmonia correlators at finite temperature. We analyze to what extent heavy quarkonia correlators are sensitive to the effect of heavy quark transport and whether it is possible to constrain the heavy quark diffusion constant by lattice calculations. Preliminary lattice calculations of quarkonia correlators performed on anisotropic lattices show that they are sensitive to the effect o...

objective(s): many cancer cells show significant resistance to drugs that kill drug sensitive cancer cells and non-tumor cells and such resistance might be a consequence of the difference in metabolism. therefore, studying the metabolism of drug resistant cancer cells and comparison with drug sensitive and normal cell lines is the objective of this research. material and methods:metabolism of c...

The serotonin transporter (SERT) is the principal mechanism for terminating serotonin (5-HT) signals in the nervous system and is a site of action for a variety of psychoactive drugs including antidepressants, amphetamines, and cocaine. Here we show that human SERTs (hSERTs) and rat SERTs are capable of robust dopamine (DA) uptake through a process that differs mechanistically from 5-HT transpo...

The basolateral membrane Na+ and Cl(-)-dependent acid-base transport processes were studied in the isolated perfused rabbit S3 proximal straight tubule. Intracellular pH (pHi) was measured with 2'7'-biscarboxyethyl-5,6-carboxyfluorescein (BCECF) and a microfluorometer coupled to the tubule perfusion apparatus. Reduction of basolateral HCO3- from 25 to 5 mM caused pHi to decrease at a rate of 0....

The transient storage model (TSM) has been widely used in studies of stream solute transport and fate, with an increasing emphasis on reactive solute transport. In this study we perform sensitivity analyses of a conservative TSM and two different reactive solute transport models (RSTM), one that includes first-order decay in the stream and the storage zone, and a second that considers sorption ...

The in vitro transport of L-p-(di-2-Chloroethylamino)[14C]phe nylalanine by i.p. grown mouse L1210 leukemias has been studied in melphalan-sensitive and melphalan-resistant cell populations. As has been reported previously, uptake of the drug is a temperature-sensitive, partly sodium-dependent, ac tive process with cell:medium accumulation in excess of 5:1 at 370 and a process which is competit...