Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for e...

The emergence of new respiratory viruses including: the Severe Acute Respiratory Syndrome; the coronavirus in 2003 (SARS-CoV); influenza A(H1N1)pdm09, in the human population in the past ten years; the emergence of a new coronavirus identified in a patient presenting severe acute respiratory syndrome who travelled to Saudi Arabia (June, 2012), denominated as Middle East Respiratory Syndrome (ME...

Coronavirus disease 2019 (COVID-19) is a disease caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) infection that was first reported on December 31, 2019 in China . This disease behaves variously: from asymptomatic infection to influenza-like symptoms and severe acute respiratory distress syndrome (ARDS) . In the recent months, COVID-19 has infected millions and killed hundre...

The 3' untranslated region (3' UTR) of the genome of the severe acute respiratory syndrome coronavirus can functionally replace its counterpart in the prototype group 2 coronavirus mouse hepatitis virus (MHV). By contrast, the 3' UTRs of representative group 1 or group 3 coronaviruses cannot operate as substitutes for the MHV 3' UTR.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a member of diverse family enveloped, nonsegmented RNA viruses. The genomic unusually large, the polymera...

Since late 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease 2019 (COVID-19) have become a worldwide threat to public health [...]