(Running title): overview of silver staining methods for proteins ABSTRACT On the basis of the physico-chemical principles underlying silver staining of proteins, which are recalled in this paper, several methods of silver staining of proteins after SDS electrophoresis in polyacrylamide gels or isoelectric focusing were tested. The most valuable protocols are presented in this report, including...

venom with serum and platelets resulted in a progressive increase in coagulant activity. Peden & Peacock (1958) found that ox serum was particularly active in potentiating the coagulant activity of the venom, and suggested that the venom acted enzymically on a substrate contained in the serum. The serum factor can be partially purified by adsorption on barium sulphate and elution by 0-2m-citrat...

Aerodynamically focused nanoparticle (AFN) printing was demonstrated for direct patterning of the solvent-free and inorganic nanoparticles. The fast excitation-purge control technique was proposed and investigated by examining the aerodynamic focusing of nanoparticles and their time-scale, with the analytical and experimental approaches. A series of direct patterning examples were demonstrated ...

The size of human factor VIII-related protein in plasma has been determined by sodium dodecyl sulfate (SDS) glyoxyl agarose electrophoresis. The protein was immobilized after the electrophoresis by coupling it to the modified agarose, and it was identified by autoradiography using purified rabbit anti-factor VIII-related antigen (VIIR:Ag). A series of multimeric forms was identified with Mr of ...

We compare the Raman scattering properties of hybrid nanostructures consisting of Ag nanoparticles (NPs) in disordered and aligned arrangements on single-walled carbon nanotubes (SWCNTs) as a result of chemical and photoreduction methods. In the latter case, the unique structure of the very small Ag NP (from 4 to 7 nm) chains generated an extremely large mode at 969 cm(-1) that was assigned to ...

Silver complexes of poly(amidoamine) (PAMAM) dendrimers as well as different {silver−PAMAM} dendrimer nanocomposite solutions have been tested in vitro against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli bacteria, using the standard agar overlay method. Both PAMAM silver salts and nanocomposites displayed considerable antimicrobial activity without the loss of solubility...

Recently, large-scale agriculture has led to increasing crop production. To increase productivity in cropping systems, attempts have been made make nano-fertilizers and deliver them the crops by extension of nanotechnology. Hence, might be defined as nanoparticles that may directly assist supplying essential nutrients for productivity. Seed germination is first most susceptible stage plant’s gr...

The mineralization of nitrobenzene was executed using an innovative method, wherein Ag/Cu2O semiconductors stimulated by visible light irradiation were supported with persulfate anions. Batch-wise experiments performed for the evaluation effects silver metal contents impregnated, concentrations and dosages on removal efficiency. physicochemical properties fresh reacted illustrated X-ray diffrac...

Background: According to the poison center data for most countries, more than thousands of people’s exposure aspirin or salicylate-containing products. So, this work aimed was offer a rapid colorimetric method monitoring concentration in exhaled breath condensate (EBC). Methods: A based on redox reaction catalyzed by nanoparticles validated analysis aspirin. 3,3 ,5,5 –Tetramethyl benzidine /H2 ...