Biomphalaria glabrata is one of the snail intermediate hosts Schistosoma mansoni , causative agent intestinal schistosomiasis disease. Numerous molecular studies using comparative approaches between susceptible and resistant snails to S. infection have helped identify numerous key candidates supporting such susceptible/resistant status. The functional approach RNA interference (RNAi) remains cr...

Nucleic acid-based therapy with plasmid DNA (pDNA) and small interfering RNA (siRNA) have received recent attention for their ability to modulate the cellular expression of genes proteins. Polyethylene glycol-modified (PEGylated) cationic nanoparticles been used as non-viral vectors in vivo delivery these nucleic acids. We reported that PEGylated liposomes (PCL) including pDNA or siRNA induce a...

RNA(i) interference is a gene silencing mechanism triggered by double-stranded (ds)RNA, which promises to contribute species-specific insect pest control strategies. The first step toward the application of RNAi as an insecticide enable efficient upon dsRNA oral delivery. desert locust, Schistocerca gregaria devastating agricultural pest. While this species responsive delivered intra-hemocoelic...

Tailored end-group modifications and the formulation of cell-penetrating urea-based foldamers enable efficient siRNA delivery into cells.

Successful delivery of irf5-siRNA by PEI-LBL-NCs resulted macrophages phenotype change from the M1 to M2 and accelerated wound healing.

SUMMARY To facilitate the designing process for vector-based siRNA and siRNA cassette, a tool set has been developed consisting of a siRNA target finder, a siRNA construct builder and a siRNA sequence scrambler. The siRNA target finder is used to identify candidate siRNA target sites. The program automates homology filtering, minimizes non-specific cross-reaction, filters target sites based on ...